Gene Rv3174
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Function unknown; involved in cellular metabolism. |
| Product | Probable short-chain dehydrogenase/reductase |
| Comments | Rv3174, (MTV014.18), len: 235 aa. Probable oxidoreductase short-chain dehyrogenase/reductase, similar to others e.g. Q9RPT7|sits from Streptomyces albus (223 aa), FASTA scores: opt: 654, E(): 6.1e-32, (49.3% identity in 215 aa overlap); Q9RI61|SCJ11.46 from Streptomyces coelicolor (230 aa), FASTA scores: opt: 626, E(): 2.9e-30, (50.9% identity in 224 aa overlap); Q9A5Z1|CC2306 from Caulobacter crescentus (252 aa), FASTA scores: opt: 430, E(): 1.3e-18, (39.45% identity in 228 aa overlap); Q51641 insect-type dehydrogenase (249 aa), FASTA scores: opt: 301, E(): 5.7e-11, (38.3% identity in 188 aa overlap); Q9HXC9|PA3883 from Pseudomonas aeruginosa (276 aa), FASTA scores: opt: 296, E(): 1.2e-10, (29.55% identity in 247 aa overlap); etc. May belong to the short-chain dehydrogenases/reductases (SDR) family. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). |
| Functional category | Intermediary metabolism and respiration |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3542138 | 3542845 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3174|Rv3174
MTSLAERTVLVTGANRGMGREYVAQLLGRKVAKVYAATRNPLAIDVSDPRVIPLQLDVTDAVSVAEAADLATDVGILINNAGISRASSVLDKDTSALRGELETNLFGPLALASAFADRIAERSGAIVNVSSVLAWLPLGMSYGVSKAAMWSATESMRIELAPRGVQVVGVYVGLVDTDMGRFADAPKSDPADVVRQVLDGIEAGKEDVLADEMSRQVRASLNVPARERIARLMGN
Bibliography
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Becq J, Gutierrez MC, Rosas-Magallanes V, Rauzier J, Gicquel B, Neyrolles O and Deschavanne P [2007]. Contribution of horizontally acquired genomic islands to the evolution of the tubercle bacilli. Sequence
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
