Gene Rv3175
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Function unknown; involved in cellular metabolism. |
| Product | Possible amidase (aminohydrolase) |
| Comments | Rv3175, (MTV014.19), len: 495 aa. Possible amidase , similar to others e.g. Q9F6D0|ZHUL enantiomer selective amidase from Streptomyces sp. R1128 (507 aa), FASTA scores: opt: 1328 ,E(): 7.5e-69, (44.5% identity in 492 aa overlap); BAB51815|MLR5350 probable amidase from Rhizobium loti (Mesorhizobium loti) (457 aa), FASTA scores: opt: 7487, E(): 1.3e-35, (35.9% identity in 482 aa overlap); O28325|YJ54_ARCFU|AF1954 putative amidase from Archaeoglobus fulgidus (453 aa), FASTA scores: opt: 532, E(): 3.2e-23, (32.05% identity in 471 aa overlap); etc. But also similar to glutamyl-tRNA amidotransferases who belong to amidase family e.g. Q9RTA9|DR1856 glutamyl-tRNA(GLN) amidotransferase, subunit A from Deinococcus radiodurans (482 aa), FASTA scores: opt: 560, E(): 8.2e-25, (30.6% identity in 513 aa overlap); Q9LCX3|GATA GLU/asp-tRNA amidotransferase subunit A from Thermus aquaticus (subsp. thermophilus) (471 aa), FASTA scores: opt: 558, E(): 1.1e-24, (30.85% identity in 486 aa overlap); Q49091|GATA_MORCA glutamyl-tRNA(GLN) amidotransferase subunit A from Moraxella catarrhalis (492 aa), FASTA scores: opt: 526, E(): 7.5e-23, (30.45% identity in 473 aa overlap); etc. Seems to belong to the amidase family. Contains PS00017 ATP/GTP-binding site motif A (P-loop). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). |
| Functional category | Intermediary metabolism and respiration |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3542860 | 3544347 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3175|Rv3175
MAMSAKASDDIAWLPATAQLAVLAAKKVSSAELVELYLSRIDTYNASLNAIVTVDPDAARRVAKRSDAARARGDELGPLHGLPITVKDSYETAGMRTTCGRRDLADYVPTQDAEAVARLRRAGAIIMGKTNMPTGNQDVQASNPVFGRTNNPWDAARTSGGSAGGGAAATAAGLTSFDYGSEIGGSTRIPAHYCGLYGHKSTWRSVPLVGHIPSAPGNPGRWGQADMACAGVQVRGARDIIPALEATVGPMRADGGFSYALAPPRAGALKDFRVAVWAEDPHCPIDADVRRAMDDAVAALRAAGAHVVEQPATIPVDMAVSHNIFQSLVFGAFAVDRSTLSPASAAALGLRAVRHPRGEAANALGATLQSHRAWLFADAARHEMRDRWAGFFNEFDVLLLPVTPTPAPLHHNKDHDRLGRTIDVDGVSRSYWDQLKWNALANIAGTPATTMPITTTATGLPIGIQAMGPAGGDRTTVEFAALLTEVLGGFRVPPL
Bibliography
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Becq J, Gutierrez MC, Rosas-Magallanes V, Rauzier J, Gicquel B, Neyrolles O and Deschavanne P [2007]. Contribution of horizontally acquired genomic islands to the evolution of the tubercle bacilli. Sequence
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
