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virulence, detoxification, adaptation
information pathways
cell wall and cell processes
stable RNAs
insertion seqs and phages
PE/PPE
intermediary metabolism and respiration
unknown
regulatory proteins
conserved hypotheticals
lipid metabolism
pseudogenes
General annotation
TypeCDS
FunctionThought to be involved in fatty acid metabolism. Generates octanol and oxidized rubredoxin from octane and reduced rubredoxin. Also hydroxylates fatty acids in the omega-position [catalytic activity: octane + reduced rubredoxin + (O)2 = 1-octanol + oxidized rubredoxin + H(2)O].
ProductProbable transmembrane alkane 1-monooxygenase AlkB (alkane 1-hydroxylase) (lauric acid omega-hydroxylase) (omega-hydroxylase) (fatty acid omega-hydroxylase) (alkane hydroxylase-rubredoxin)
CommentsRv3252c, (MTCY20B11.27c), len: 416 aa. Probable alkB, transmembrane alkane-1-monooxygenase, highly similar to many (see Marin et al., 2001) e.g. Q9AE68|ALKB2 from Rhodococcus erythropolis (408 aa), FASTA scores: opt: 2018, E(): 9.6e-122, (68.6% identity in 415 aa overlap); Q9AFD5|ALKB from Nocardioides sp. CF8 (483 aa), FASTA scores: opt: 1485, E(): 1.4e-87, (56.55% identity in 405 aa overlap); Q9XAU0|ALKB1 from Rhodococcus erythropolis (391 aa), FASTA scores: opt: 1400, E(): 3.3e-82, (62.6% identity in 396 aa overlap); Q9XBM1|ALKB from Prauserella rugosa (490 aa), FASTA scores: opt: 1266, E(): 1.5e-73, (57.55% identity in 410 aa overlap); CAC40954|ALKB4 from Rhodococcus erythropolis (386 aa), FASTA scores: opt: 1190, E(): 9.1e-69, (54.3% identity in 383 aa overlap); etc.
Functional categoryLipid metabolism
TranscriptomicsmRNA identified by microarray analysis and up-regulated after 24h of starvation (see Betts et al., 2002).
MutantDisruption of this gene provides a growth advantage for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv and CDC1551 strains (see Sassetti et al., 2003 and Lamichhane et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Non essential gene for growth in vitro by Tn5370 transposon mutagenesis of H37Rv strain but mutant is more virulent in SCID mice (see McAdam et al., 2002).
Check for mutants available at TARGET website
Coordinates
TypeStartEndOrientation
CDS36307383631988-
Genomic sequence
Feature type Upstream flanking region (bp) Downstream flanking region (bp) Update
       
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3252c|alkB
MTTQIGSGGPEAPRPPEVEEWRDKKRYLWLMGLIAPTALVVMLPLIWGMNQLGWHAAAQVPLWIGPILLYVLLPLLDLRFGPDGQNPPDEVTDRLENDKYYRYCTYIYIPFQYLSVVLGAYLFTAANLSWLGFDGALSWAGKLGVALSVGVLGGVGINTAHEMGHKKDSLERWLSKITLAQTCYGHFYIEHNRGHHVRVSTPEDPASARFGETLWEFLPRSVIGGLRSAVHLEAQRLRRLGVSPWNPMTYLRNDVLNAWLMSVVLWGGLIAVFGPALIPFVIIQAVFGFSLLEAVNYLEHYGLLRQKSANGRYERCAPVHSWNSDHIVTNLFLYHLQRHSDHHANPTRRYQTLRSMAGAPNLPSGYASMISLTYFPPLWRKVMDHRVLEHYGGDITRVNLHPRVREKALARYGASA