Gene Rv3253c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Thought to be involved in cationic amino acid transport across the membrane. Responsible for the translocation of the substrate across the membrane. |
| Product | Possible cationic amino acid transport integral membrane protein |
| Comments | Rv3253c, (MTCY20B11.28c), len: 495 aa. Possible cationic amino acid transporter, integral membrane protein, similar to many e.g. O69844|SC1C3.02 putative cationic amino acid transporter from Streptomyces coelicolor (503 aa), FASTA scores: opt: 1649, E(): 5.8e-92, (52.6% identity in 485 aa overlap); Q9AE69 putative transporter (fragment) from Rhodococcus erythropolis (385 aa), FASTA scores: opt: 1594, E(): 9.7e-89, (62.0% identity in 387 aa overlap); Q9PBD7|XF2207 cationic amino acid transporter from Xylella fastidiosa (483 aa), FASTA scores: opt: 1079, E(): 1.2e-57, (40.55% identity in 493 aa overlap); Q9SRU9|F20H23.25 putative cationic amino acid transporter from Arabidopsis thaliana (Mouse-ear cress) (614 aa), FASTA scores: opt: 802, E(): 6.7e-41, (36.4% identity in 445 aa overlap); P30823|CTR1_RAT|SLC7A1|ATRC1 high-affinity cationic amino acid transporter-1 from Rattus norvegicus (Rat) (624 aa), FASTA scores: opt: 782, E(): 1.1e-39, (36.1% identity in 432 aa overlap); etc. Relatives in Mycobacterium tuberculosis include: MTCY3G12.14, E(): 5.6e-31; MTCY39.19, E(): 1.6e-14. Seems to belong to the APC family. |
| Functional category | Cell wall and cell processes |
| Proteomics | Identified by mass spectrometry in M. tuberculosis H37Rv-infected guinea pig lungs at 90 days but not 30 days (See Kruh et al., 2010). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3632097 | 3633584 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3253c|Rv3253c
MAGRRRMKSVEQSIADTDEPTTRLRKDLTWWDLVVFGVSVVIGAGIFTVTASTAGDITGPAIWISFLIAAATCALAALCYAEFASTLPVAGSAYTFSYATFGEFLAWVIGWNLVLELAMGAAVVAKGWSSYLGTVFGFGNGTGHLGSLQLDWGALVIVTLVATLIALGTKLSSRFSAVVTAIKVSVVVLVVVVGAFYIRAANYSPFIPEPEVQHHGGGLDQSVFSLLTGAQGSHYGWYGVLAGASIVFFAFIGFDIVATMAEETKRPQRDVPRGILASLGVVTLLYVAVSVVLSGMVPYTQLRTVPGRGPANLATAFQANGVYWASGIISVGALAGLTTVVMVLMLGQCRVLFAMARDGLVPRQLAKTGSRGTPVRVTVLVAVLVATTASVFPITKLEEMVNVGTLFAFILVSAGVVVLRRTRPDLQRGFTAPWVPLLPIAAVCACLWLMLNLTALTWIRFGIWLVAGTAIYVGYGRRHSAQGLRQARESATRRC
Bibliography
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Rodrigue S et al. [2007]. Identification of mycobacterial sigma factor binding sites by chromatin immunoprecipitation assays. Regulon
- Kruh NA et al. [2010]. Portrait of a pathogen: the Mycobacterium tuberculosis proteome in vivo. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
