Gene Rv3254
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Function unknown |
| Product | Conserved hypothetical protein |
| Comments | Rv3254, (MTCY20B11.29), len: 462 aa. Conserved hypothetical protein, similar to CAC37877|SC1G7.02 putative secreted protein from Streptomyces coelicolor (440 aa), FASTA scores: opt: 606, E(): 6.2e-31, (31.7% identity in 445 aa overlap); O86550|SC1F2.13c hypothetical 50.7 KDA protein from Streptomyces coelicolor (476 aa), FASTA scores: opt: 577, E(): 4.5e-29, (32.5% identity in 400 aa overlap); Q9L0A8|SCC24.09 putative secreted protein from Streptomyces coelicolor (468 aa), FASTA scores: opt: 380, E(): 1.3e-16, (30.7% identity in 391 aa overlap); BAB48792|MLL1411 probable FAD-dependent monooxygenase from Rhizobium loti (Mesorhizobium loti) (421 aa), FASTA scores: opt: 128, E(): 1.1, (25.2% identity in 397 aa overlap); Q9L7X9|BENF benzoate-specific porin-like protein from Pseudomonas putida (397 aa), FASTA scores: opt: 119, E(): 4, (24.85% identity in 157 aa overlap); etc. Also similar to N-terminus of AAK46259|MT1987 putative ferredoxin reductase, electron transfer component from Mycobacterium tuberculosis strain CDC1551 (839 aa), FASTA scores: opt: 493, E(): 1.5e-23, (30.65% identity in 382 aa overlap). |
| Functional category | Conserved hypotheticals |
| Proteomics | Identified in the cytosol, cell wall, and cell membrane fractions of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). |
| Transcriptomics | mRNA identified by microarray analysis and up-regulated after 24h of starvation (see citation below). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv and CDC1551 strains (see Sassetti et al., 2003 and Lamichhane et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3633675 | 3635063 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3254|Rv3254
VVIGASIAGLCAARVLSDFYSTVTVFERDELPEAPANRATVPQDRHLHMLMARGAQEFDSLFPGLLHDMVAAGVPMLENRPDCIYLGAAGHVLGTGHTLRKEFTAYVPSRPHLEWQLRRRVLQLSNVQIVRRLVTEPQFERRQQRVVGVLLDSPGSGQDREREEFIAADLVVDAAGRGTRLPVWLTQWGYRRPAEDTVDIGISYASHQFRIPDGLIAEKVVVAGASHDQSLGLGMLCYEDGTWVLTTFGVADAKPPPTFDEMRALADKLLPARFTAALAQAQPIGCPAFHAFPASRWRRYDKLERFPRGIVPFGDAVASFNPTFGQGMTMTSLQAGHLRRALKARNSAMKGDLAAELNRATAKTTYPVWMMNAIGDISFHHATAEPLPRWWRPAGSLFDQFLGAAETDPVLAEWFLRRFSLLDSLYMVPSVPIIGRAIAHNLRLWLKEQRERRQPVTTRRSP
Bibliography
- Betts JC et al. [2002]. Evaluation of a nutrient starvation model of Mycobacterium tuberculosis persistence by gene and protein expression profiling. Transcriptome
- Lamichhane G et al. [2003]. A postgenomic method for predicting essential genes at subsaturation levels of mutagenesis: application to Mycobacterium tuberculosis. Mutant
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Mawuenyega KG et al. [2005]. Mycobacterium tuberculosis functional network analysis by global subcellular protein profiling. Proteomics
- Rodrigue S et al. [2007]. Identification of mycobacterial sigma factor binding sites by chromatin immunoprecipitation assays. Regulon
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
