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virulence, detoxification, adaptation
information pathways
cell wall and cell processes
stable RNAs
insertion seqs and phages
PE/PPE
intermediary metabolism and respiration
unknown
regulatory proteins
conserved hypotheticals
lipid metabolism
pseudogenes
General annotation
TypeCDS
FunctionInvolved in transcriptional regulation of active transport of inorganic phosphate across the membrane.
ProductProbable phosphate-transport system transcriptional regulatory protein PhoU homolog 1 PhoY1
CommentsRv3301c, (MTCI418A.03c), len: 221 aa. Probable phoY1, phosphate-transport system regulatory protein, highly similar to Q50047|phoY|PHOU1|PHOY1|ML2188 phosphate transport system protein PHOU homolog 1 from Mycobacterium leprae (222 aa), FASTA scores: opt: 929, E(): 7.8e-51, (61.45% identity in 218 aa overlap). Also highly similar to Q9FCE2|2SCD46.42c putative regulatory protein (fragment) from Streptomyces coelicolor (123 aa), FASTA scores: opt: 324, E(): 1.8e-13, (43.65% identity in 103 aa overlap); Q9L0R3|SCD8A.01c putative phosphate transport system regulatory protein (fragment) from Streptomyces coelicolor (139 aa), FASTA scores: opt: 309, E(): 1.7e-12, (36.7% identity in 139 aa overlap); Q52989|PHOU_RHIME phosphate transport system protein from Rhizobium meliloti (Sinorhizobium meliloti) (237 aa), FASTA scores: opt: 292, E(): 3.1e-11, (26.3% identity in 213 aa overlap); etc. And highly similar to Mycobacterium tuberculosis O53833|PHU2_MYCTU|MTV043_13c|PHOU2|PHOY2|Rv0821c|MT0843 phosphate transport system protein PHOU homolog 2 (213 aa) (63.4% identity in 213 aa overlap). Belongs to the PHOU family.
Functional categoryCell wall and cell processes
ProteomicsIdentified in the membrane fraction of M. tuberculosis H37Rv using 1D-SDS-PAGE and uLC-MS/MS (See Gu et al., 2003). Identified in the cytosol of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). Identified in the membrane fraction of M. tuberculosis H37Rv using nanoLC-MS/MS (See Xiong et al., 2005). Identified by mass spectrometry in whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate or membrane protein fraction (See de Souza et al., 2011).
MutantNon-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011).
Check for mutants available at TARGET website
Coordinates
TypeStartEndOrientation
CDS36869123687577-
Genomic sequence
Feature type Upstream flanking region (bp) Downstream flanking region (bp) Update
       
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3301c|phoY1
MRTVYHQRLTELAGRLGEMCSLAGIAMKRATQALLEADIGAAEQVIRDHERIVAMRAQVEKEAFALLALQHPVAGELREIFSAVQIIADTERMGALAVHIAKITRREYPNQVLPEEVRNCFADMAKVAIALGDSARQVLVNRDPQEAAQLHDRDDAMDDLHRHLLSVLIDREWRHGVRVGVETALLGRFFERFADHAVEVGRRVIFMVTGVLPTEDEISTY