Gene Rv3327
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Involved in the transposition in the insertion sequence element IS1547. |
| Product | Probable transposase fusion protein |
| Comments | Rv3327, (MTV016.27), len: 570 aa. Probable fusion protein. Indeed, N-terminal part corresponds to entire O07269 transposase of IS1547 (383 aa), and C-terminal part identical to MTCI249B.03c (210 aa). N-terminal part is identical to MTV042_7 (188 aa); C-terminal part (aa 378-570) is similar to hypothetical 20.5 kDa protein from Escherichia coli P76222|YNJA_ECOLI (182 aa), FASTA scores: opt: 292, E(): 5.3e-11, (32.6% identity in 181 aa overlap). |
| Functional category | Insertion seqs and phages |
| Proteomics | Identified by mass spectrometry in whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate or membrane protein fraction (See de Souza et al., 2011). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv and CDC1551 strains (see Sassetti et al., 2003 and Lamichhane et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3711749 | 3713461 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3327|Rv3327
MVVVGTDAHKYSHTFVATDEVGRQLGEKTVKATTAGHATAIMWAREQFGLELIWGIEDCRNMSARLERDLLAAGQQVVRVPTKLMAQTRKSARSRGKSDPIDALAVARAVLRETDLPLATHDETSRELKLLTDRRDVLVAQRTSAINRLRWLVHELDPERAPAARSLDAAKHQQALRTWLDTQPGLVAELARAELTDIIRLTGEINTLAQRISARVHQVAPALLEIPGCAELTAAKIVGEAAGVTRFKSEAAFACHAAVAPIPVWSGNTAGQMRLSRSGNRQLNAALHRIALTQIRMTDSRGQAYYQRLQDAGKTKRAALRCLKRRLARTVFQALRTVHQPSSEHTQPAAACHRSYCSSHLGEPPRLTDMTQKTRIQPLPPKRAGLLIRALYRIAKRRFGEVPEPFTVTAHHRRLLIANVVHEALLQRASRKLPPSVRELAVFWTARSIGCSWCVDFGAMLQRLDGLDVDRLTDIDNYATSSKFSDDERAAIAYAEAMTADPHSVTDEQVADLRARFGEAGVIELTYQIGVENMRARMNSALGITEQGFNSGDACRVPWAAPDVPSAESR
Bibliography
- Lamichhane G et al. [2003]. A postgenomic method for predicting essential genes at subsaturation levels of mutagenesis: application to Mycobacterium tuberculosis. Mutant
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
