Gene Rv3329
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Function unknown; probably involved in cellular metabolism. |
| Product | Probable aminotransferase |
| Comments | Rv3329, (MTV016.29), len: 438 aa (start uncertain). Probable aminotransferase, similar to many e.g. O86744|SC6A9.12 from Streptomyces coelicolor (457 aa), FASTA scores: opt: 2120, E(): 5.1e-125, (70.1% identity in 438 aa overlap); Q9I6J2|PA0299 from Pseudomonas aeruginosa (456 aa), FASTA scores: opt: 983, E(): 5.7e-54, (38.1% identity in 425 aa overlap); Q53196|Y4UB_RHISN from Rhizobium sp. strain NGR234 plasmid sym pNGR234a (467 aa), FASTA scores: opt: 971, E(): 3.3e-53, (39.25% identity in 438 aa overlap); P33189|YHXA_BACSU from Bacillus subtilis (450 aa), FASTA scores: opt: 933, E(): 7.5e-51, (40.25% identity in 435 aa overlap); etc. Equivalent to AAK47775 from Mycobacterium tuberculosis strain CDC1551 (466 aa) but shorter 28 aa. Cofactor: pyridoxal phosphate. Could belong to class-III of pyridoxal-phosphate-dependent aminotransferases. |
| Functional category | Intermediary metabolism and respiration |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3714392 | 3715708 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3329|Rv3329
LHFARHGAGIQHPVIVRGDGVTIFDDRGKSYLDALSGLFVVQVGYGRAELAEAAARQAGTLGYFPLWGYATPPAIELAERLARYAPGDLNRVFFTSGGTEAVETAWKVAKQYFKLTGKPGKQKVISRSIAYHGTTQGALAITGLPLFKAPFEPLTPGGFRVPNTNFYRAPLHTDLKEFGRWAADRIAEAIEFEGPDTVAAVFLEPVQNAGGCIPAPPGYFERVREICDRYDVLLVSDEVICAFGRIGSMFACEDLGYVPDMITCAKGLTSGYSPLGAMIASDRLFEPFNDGETMFAHGYTFGGHPVSAAVGLANLDIFEREGLSDHVKRNSPALRATLEKLYDLPIVGDIRGEGYFFGIELVKDQATKQTFTDDERARLLGQVSAALFEAGLYCRTDDRGDPVVQVAPPLISGQPEFDTIETILRSVLTDTGRKYLHL
Bibliography
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
