Gene Rv3340
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Transforms O-acetylhomoserine into L-methionine [catalytic activity: O-acetyl-L-homoserine + methanethiol = L-methionine + acetate]. |
| Product | Probable O-acetylhomoserine sulfhydrylase MetC (homocysteine synthase) (O-acetylhomoserine (thiol)-lyase) (OAH sulfhydrylase) (O-acetyl-L-homoserine sulfhydrylase) |
| Comments | Rv3340, (MTV016.40), len: 449 aa. Probable metC, O-acetyl-L-homoserine sulfhydrylase, highly similar to many e.g. Q9K9P2|BH2603 O-acetylhomoserine sulfhydrylase from Bacillus halodurans (430 aa), FASTA scores: opt: 1716, E(): 3.3e-97, (60.45% identity in 425 aa overlap); Q9HUE4|METY|PA5025 homocysteine synthase from Pseudomonas aeruginosa (425 aa), FASTA scores: opt: 1517, E(): 4.4e-85, (56.95% identity in 425 aa overlap); Q9WZY4|TM0882 O-acetylhomoserine sulfhydrylase from Thermotoga maritima (430 aa), FASTA scores: opt: 1488, E(): 2.6e-83, (55.75% identity in 418 aa overlap); BAB54344|MLR8465 O-acetylhomoserine sulfhydrylase from Rhizobium loti (Mesorhizobium loti) (426 aa), FASTA scores: opt: 1445, E(): 1.1e-80, (53.2% identity in 419 aa overlap); P50125|CYSD_EMENI O-acetylhomoserine (thiol)-lyase from Emericella nidulans (Aspergillus nidulans) (437 aa), FASTA scores: opt: 1442, E(): 1.7e-80, (53.7% identity in 430 aa overlap); etc. Contains PS00868 Cys/Met metabolism enzymes pyridoxal-phosphate attachment site. Cofactor: pyridoxal phosphate. Belongs to the trans-sulfuration enzymes family. |
| Functional category | Intermediary metabolism and respiration |
| Proteomics | Identified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in the membrane protein fraction and whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate (See de Souza et al., 2011). Translational start site supported by proteomics data (See Kelkar et al., 2011). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3726127 | 3727476 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3340|metC
MSADSNSTDADPTAHWSFETKQIHAGQHPDPTTNARALPIYATTSYTFDDTAHAAALFGLEIPGNIYTRIGNPTTDVVEQRIAALEGGVAALFLSSGQAAETFAILNLAGAGDHIVSSPRLYGGTYNLFHYSLAKLGIEVSFVDDPDDLDTWQAAVRPNTKAFFAETISNPQIDLLDTPAVSEVAHRNGVPLIVDNTIATPYLIQPLAQGADIVVHSATKYLGGHGAAIAGVIVDGGNFDWTQGRFPGFTTPDPSYHGVVFAELGPPAFALKARVQLLRDYGSAASPFNAFLVAQGLETLSLRIERHVANAQRVAEFLAARDDVLSVNYAGLPSSPWHERAKRLAPKGTGAVLSFELAGGIEAGKAFVNALKLHSHVANIGDVRSLVIHPASTTHAQLSPAEQLATGVSPGLVRLAVGIEGIDDILADLELGFAAARRFSADPQSVAAF
Bibliography
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Målen H et al. [2010]. Definition of novel cell envelope associated proteins in Triton X-114 extracts of Mycobacterium tuberculosis H37Rv. Proteomics
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- Kelkar DS et al. [2011]. Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry. Proteomics Sequence
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
