Gene Rv3376
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Function unknown |
| Product | Conserved hypothetical protein |
| Comments | Rv3376, (MTV004.34), len: 217 aa. Hypothetical protein, similar to various bacterial proteins (notably hydrolases) e.g. Q9RUP0|DR1344 hydrolase from Deinococcus radiodurans (222 aa), FASTA scores: opt: 348, E(): 1.8e-15, (36.75% identity in 215 aa overlap); Q9RXA1|DR0414 hydrolase (CBBY/CBBZ/GPH/YIEH family) from Deinococcus radiodurans (155 aa), FASTA scores: opt: 233, E(): 3.5e-08, (36.4% identity in 151 aa overlap); Q9X0Q9|TM1177 conserved hypothetical protein from Thermotoga maritima (225 aa), FASTA scores: opt: 231, E(): 6.6e-08, (27.6% identity in 221 aa overlap); Q9ABI3|CC0244 hydrolase, haloacid dehalogenase-like from Caulobacter crescentus (213 aa), FASTA scores: opt: 213, E(): 9.1e-07, (28.95% identity in 221 aa overlap); BAB38231|ECS4808 putative phosphatase from Escherichia coli strain O157:H7 (206 aa), FASTA scores: opt: 210, E(): 1.4e-06, (26.95% identity in 193 aa overlap); etc. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). |
| Functional category | Conserved hypotheticals |
| Proteomics | Identified by mass spectrometry in whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate or membrane protein fraction (See de Souza et al., 2011). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Required for survival in primary murine macrophages, by transposon site hybridization (TraSH) in H37Rv (See Rengarajan et al., 2005). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3790156 | 3790809 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3376|Rv3376
LSISAVVFDRDGVLTSFDWTRAEEDVRRITGLPLEEIERRWGGWLNGLTIDDAFVETQPISEFLSSLARELELGSKARDELVRLDYMAFAQGYPDARPALEEARRRGLKVGVLTNNSLLVSARSLLQCAALHDLVDVVLSSQMIGAAKPDPRAYQAIAEALGVSTTSCLFFDDIADWVEGARCAGMRAYLVDRSGQTRDGVVRDLSSLGAILDGAGP
Bibliography
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Rengarajan J et al. [2005]. Genome-wide requirements for Mycobacterium tuberculosis adaptation and survival in macrophages. Mutant
- Becq J, Gutierrez MC, Rosas-Magallanes V, Rauzier J, Gicquel B, Neyrolles O and Deschavanne P [2007]. Contribution of horizontally acquired genomic islands to the evolution of the tubercle bacilli. Sequence
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- Mazandu GK et al. [2012]. Function prediction and analysis of mycobacterium tuberculosis hypothetical proteins. Function
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
