Gene Rv3402c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Function unknown; thought to be involved in cell process. |
| Product | Conserved hypothetical protein |
| Comments | Rv3402c, (MTCY78.26), len: 412 aa. Conserved hypothetical protein, probably involved in cell process, similar to various proteins generally involved in extracellular compounds (lipopolysaccharide O-antigen) biosynthesis e.g. O68392|RFBE perosamine synthetase from Brucella melitensis (367 aa), FASTA scores: opt: 420, E(): 1.2e-19, (26.15% identity in 375 aa overlap); Q9L6C1 3,4-dehydratase-like protein from Streptomyces antibioticus (393 aa), FASTA scores: opt: 419, E(): 1.5e-19, (30.65% identity in 385 aa overlap); Q9RR26|OLENI dehydratase from Streptomyces antibioticus (393 aa), FASTA scores: opt: 416, E(): 2.3e-19, (30.65% identity in 385 aa overlap); O33942 eryciv protein from Saccharopolyspora erythraea (Streptomyces erythraeus) (401 aa), FASTA scores: opt: 410, E(): 5.6e-19, (31.75% identity in 362 aa overlap); Q9UZI4|ASPB-LIKE1|PAB0774 aspartate aminotransferase (ASPB-LIKE1) from Pyrococcus abyssi (366 aa), FASTA scores: opt: 402, E(): 1.7e-18, (27.05% identity in 377 aa overlap); O88001|WLBC putative amino-sugar biosynthesis protein from Bordetella bronchiseptica (Alcaligenes bronchisepticus) (366 aa), FASTA scores: opt: 394, E(): 5.6e-18, (26.8% identity in 347 aa overlap); Q45378|BPLC DNA for lipopolysaccharide biosynthesis from Bordetella pertussis (366 aa), FASTA scores: opt: 393, E(): 6.5e-18, (26.8% identity in 347 aa overlap); etc. |
| Functional category | Cell wall and cell processes |
| Proteomics | Predicted secreted protein - identified in culture filtrates of M. tuberculosis H37Rv; signal peptide predicted (See Malen et al., 2007). |
| Transcriptomics | DNA microarrays indicate repression by iron and IdeR|Rv2711 in M. tuberculosis H37Rv (See Rodriguez et al., 2002). DNA microarrays show lower level of expression in M. tuberculosis H37Rv than in phoP|Rv0757 mutant (See Walters et al., 2006). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Disruption of this gene provides a growth advantage for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv and CDC1551 strains (see Sassetti et al., 2003 and Lamichhane et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3820653 | 3821891 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3402c|Rv3402c
MKIRTLSGSVLEPPSAVRATPGTSMLKLEPGGSTIPKIPFIRPSFPGPAELAEDFVQIAQANWYTNFGPNERRFARALRDYLGPHLHVATLANGTLALLAALHVSFGAGTRDRYLLMPSFTFVGVAQAALWTGYRPWFIDIDANTWQPCVHSARAVIERFRDRIAGILLANVFGVGNPQISVWEELAAEWELPIVLDSAAGFGSTYADGERLGGRGACEIFSFHATKPFAVGEGGALVSRDPRLVEHAYKFQNFGLVQTRESIQLGMNGKLSEISAAIGLRQLVGLDRRLASRRKVLECYRTGMADAGVRFQDNANVASLCFASACCTSADHKAAVLGSLRRHAIEARDYYNPPQHRHPYFVTNAELVESTDLAVTADICSRIVSLPVHDHMAPDDVARVVAAVQEAEVRGE
Bibliography
- Gold B et al. [2001]. The Mycobacterium tuberculosis IdeR is a dual functional regulator that controls transcription of genes involved in iron acquisition, iron storage and survival in macrophages. Regulon
- Rodriguez GM, Voskuil MI, Gold B, Schoolnik GK and Smith I [2002]. ideR, An essential gene in mycobacterium tuberculosis: role of IdeR in iron-dependent gene expression, iron metabolism, and oxidative stress response. Transcriptome
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Lamichhane G et al. [2003]. A postgenomic method for predicting essential genes at subsaturation levels of mutagenesis: application to Mycobacterium tuberculosis. Mutant
- Manabe YC et al. [2005]. Both Corynebacterium diphtheriae DtxR(E175K) and Mycobacterium tuberculosis IdeR(D177K) are dominant positive repressors of IdeR-regulated genes in M. tuberculosis. Regulon
- Walters SB et al. [2006]. The Mycobacterium tuberculosis PhoPR two-component system regulates genes essential for virulence and complex lipid biosynthesis. Transcriptome
- Målen H et al. [2007]. Comprehensive analysis of exported proteins from Mycobacterium tuberculosis H37Rv. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
