Gene Rv3403c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Unknown |
| Product | Hypothetical protein |
| Comments | Rv3403c, (MTCY78.25), len: 533 aa. Hypothetical unknown protein, but some weak similarity to Q9KJP2 hypothetical 54.9 KDA protein from Myxococcus xanthus (504 aa), FASTA scores: opt: 157, E(): 0.011, (24.1% identity in 548 aa overlap). |
| Functional category | Conserved hypotheticals |
| Proteomics | Identified by mass spectrometry in M. tuberculosis H37Rv-infected guinea pig lungs at 30 days but not 90 days (See Kruh et al., 2010). |
| Transcriptomics | mRNA identified by microarray analysis and down-regulated after 4h, 24h and 96h of starvation (see citation below). DNA microarrays indicate repression by iron and IdeR|Rv2711 in M. tuberculosis H37Rv (See Rodriguez et al., 2002). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Disruption of this gene provides a growth advantage for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv and CDC1551 strains (see Sassetti et al., 2003 and Lamichhane et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3822262 | 3823863 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3403c|Rv3403c
MLAFPYLMTMITPPTFDVAFIGSGAACSMTLLEMADALLSSPSASPKLRIAVVERDEQFWCGIPYGQRSSIGSLAIQKLDDFADEPEKAAYRIWLEQNKQRWLAFFQAEGGAAAARWICDNRDALDGNQWGELYLPRFLFGVFLSEQMIAAIAALGERDLAEIVTIRAEAMSAHSADGHYRIGLRPSGNGPTAIAAGKVVVAIGSPPTKAILASDSEPAFTYINDFYSPGGESNVARLRDSLDRVESWEKRNVLVVGSNATSLEALYLMRHDARIRARVRSITVISRSGVLPYMICNQPPEFDFPRLRTLLCTEAIAAADLMSAIRDDLATAEERSLNLADLYDAVAALFGQALHKMDLVQQEEFFCVHGMNFTKLVRRAGRDCRQASEELAADGTLSLLAGEVLRVDACASGQPFATMTYRAAGAEHTHPVPFAAVVNCGGFEELDTCSSPFLVSAMQNGLCRPNRTNRGLLVNDDFEASPGFCVIGPLVGGNFTPKIRFWHVESAPRVRSLAKSLAASLLASLQPVALAPC
Bibliography
- Gold B et al. [2001]. The Mycobacterium tuberculosis IdeR is a dual functional regulator that controls transcription of genes involved in iron acquisition, iron storage and survival in macrophages. Regulon
- Betts JC et al. [2002]. Evaluation of a nutrient starvation model of Mycobacterium tuberculosis persistence by gene and protein expression profiling. Transcriptome
- Rodriguez GM, Voskuil MI, Gold B, Schoolnik GK and Smith I [2002]. ideR, An essential gene in mycobacterium tuberculosis: role of IdeR in iron-dependent gene expression, iron metabolism, and oxidative stress response. Transcriptome
- Lamichhane G et al. [2003]. A postgenomic method for predicting essential genes at subsaturation levels of mutagenesis: application to Mycobacterium tuberculosis. Mutant
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Prakash P et al. [2005]. Computational prediction and experimental verification of novel IdeR binding sites in the upstream sequences of Mycobacterium tuberculosis open reading frames. Regulon
- Kruh NA et al. [2010]. Portrait of a pathogen: the Mycobacterium tuberculosis proteome in vivo. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- Mazandu GK et al. [2012]. Function prediction and analysis of mycobacterium tuberculosis hypothetical proteins. Function
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
