Gene Rv3468c (rmlB3)
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Possibly involved in dTDP-L-rhamnose biosynthesis [catalytic activity: dTDP-glucose = dTDP-4-dehydro-6-deoxy-D-glucose + H(2)O]. |
| Product | Possible dTDP-glucose 4,6-dehydratase |
| Comments | Rv3468c, (MTCY13E12.21c), len: 364 aa. Possible dTDP-glucose-4,6-dehydratase, but experimental study shown that the purified protein didn't have dTDP-glucose dehydratase (rmlB) activity (see Ma et al., 2001). Similar to others e.g. O08246|MTME from Streptomyces argillaceus (331 aa), FASTA scores: opt: 238, E(): 1.2e-07, (29.65% identity in 344 aa overlap); Q9LFG7|F4P12_220 from Arabidopsis thaliana (Mouse-ear cress) (433 aa), FASTA scores: opt: 237, E(): 1.8e-07, (27.25% identity in 308 aa overlap); Q9LZI2|F26K9_260 from Arabidopsis thaliana (Mouse-ear cress) (445 aa), FASTA scores: opt: 225, E(): 1e-06, (25.95% identity in 335 aa overlap); etc. Also similar to various enzymes and hypothetical unknowns proteins e.g. BAB48655|MLL1234 UDP-glucose 4-epimerase from Rhizobium loti (Mesorhizobium loti) (307 aa), FASTA scores: opt: 757, E(): 4.6e-40, (43.4% identity in 302 aa overlap). First start taken, alternative at 17080 in cSCYY13E12 suggested by similarity. Note that previously known as rmlB3 (see Ma et al., 2001). |
| Functional category | Intermediary metabolism and respiration |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3884975 | 3886069 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3468c|Rv3468c
VGTHAATMRVRAGVRSSPLLLHAGTPPTAAAAESGMRTLVTGSSGHLGEALVRTLRARGADIVSLDSRPSRYTNIVGCVSDRALLRDVMAGVEVVFHAAAHHKPQLAFLPRQAFLDTNIIGTQTVLDAAVAANVRAFVMTSSTTVFGDALTPPADQPAAWIDESVTPIPKNIYGVTKASSEDLCQLAHRNDGLACVVLRVARFFVEGDDMPDLYDGRSQDNIKANEYACRRVALEDAVDAHLNAAQRAPQLGFGRYLVSATTPFTRDDLTQLRTDAASVFARRVPLAAAVWTQRGWRFPDRLDRVYVNSRARRDLNWRPRFDLNAVAARLARGQSVHTPLSQLVGSKAYAHSSYHRGVFAPARP
Bibliography
- Ma Y, Stern RJ, Scherman MS, Vissa VD, Yan W, Jones VC, Zhang F, Franzblau SG, Lewis WH and McNeil MR [2001]. Drug targeting Mycobacterium tuberculosis cell wall synthesis: genetics of dTDP-rhamnose synthetic enzymes and development of a microtiter plate-based screen for inhibitors of conversion of dTDP-glucose to dTDP-rhamnose. Product Biochemistry Function
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
