Gene Rv3479
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Unknown |
| Product | Possible transmembrane protein |
| Comments | Rv3479, (MTCY13E12.32), len: 1021 aa. Possible transmembrane protein, with hydrophobic stretches at C-terminus. Start changed since first submission (-54 aa). Alternative nucleotide at position 3896340 (T->G; L174R) has been observed. |
| Functional category | Cell wall and cell processes |
| Proteomics | Identified in the cell membrane fraction of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). Identified in the membrane fraction of M. tuberculosis H37Rv using nanoLC-MS/MS; predicted integral membrane protein (See Xiong et al., 2005). Identified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in M. tuberculosis H37Rv-infected guinea pig lungs at 30 days but not 90 days (See Kruh et al., 2010). Identified by mass spectrometry in the membrane protein fraction and whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate (See de Souza et al., 2011). |
| Transcriptomics | mRNA identified by microarray analysis and down-regulated after 96h of starvation (see citation below). DNA microarrays show higher level of expression in M. tuberculosis H37Rv than in phoP|Rv0757 mutant (See Walters et al., 2006). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv and CDC1551 strains (see Sassetti et al., 2003 and Lamichhane et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3895820 | 3898885 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3479|Rv3479
MAGVTREINLLAQASQWRRLGGTFPTNSQLTNESAASLRLYAQLIDLLDMVVDVDILSGTSAGGINAALLASSRVTGSDLGGIRDLWLDLGALTELLRDPRDKKTPSLLYGDERIFAALAKRLPKLATGPFPPTTFPEAARTPSTTLYITTTLLAGETSRFTDSFGTLVQDVDLRGLFTFTETDLARPDTAPALALAARSSASFPLAFEPSFLPFTKGTAKKGEVPARPAMAPFTSLTRPHWVSDGGLLDNRPIGVLFKRIFDRPARRPVRRVLLFVVPSSGPAPDPMHEPPPDNVDEPLGLIDGLLKGLAAVTTQSIAADLRAIRAHQDCMEARTDAKLRLAELAATLRNGTRLLTPSLLTDYRTREATKQAQTLTSALLRRLSTCPPESGPATESLPKSWSAELTVGGDADKVCRQQITATILLSWSQPTAQPLPQSPAELARFGQPAYDLAKGCALTVIRAAFQLARSDADIAALAEVTEAIHRAWRPTASSDLSVLVRTMCSRPAIRQGSLENAADQLAADYLQQSTVPGDAWERLGAALVNAYPTLTQLAASASADSGAPTDSLLARDHVAAGQLETYLSYLGTYPGRADDSRDAPTMAWKLFDLATTQRAMLPADAEIEQGLELVQVSADTRSLLAPDWQTAQQKLTGMRLHHFGAFYKRSWRANDWMWGRLDGAGWLVHVLLDPRRVRWIVGERADTNGPQSGAQWFLGKLKELGAPDFPSPGYPLPAVGGGPAQHLTEDMLLDELGFLDDPAKPLPASIPWTALWLSQAWQQRVLEEELDGLANTVLDPQPGKLPDWSPTSSRTWATKVLAAHPGDAKYALLNENPIAGETFASDKGSPLMAHTVAKAAATAAGAAGSVRQLPSVLKPPLITLRTLTLSGYRVVSLTKGIARSTIIAGALLLVLGVAAAIQSVTVFGVTGLIAAGTGGLLVVLGTWQVSGRLLFALLSFSVVGAVLALATPVVREWLFGTQQQPGWVGTHAYWLGAQWWHPLVVVGLIALVAIMIAAATPGRR
Bibliography
- Betts JC et al. [2002]. Evaluation of a nutrient starvation model of Mycobacterium tuberculosis persistence by gene and protein expression profiling. Transcriptome
- Lamichhane G et al. [2003]. A postgenomic method for predicting essential genes at subsaturation levels of mutagenesis: application to Mycobacterium tuberculosis. Mutant
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Xiong Y, Chalmers MJ, Gao FP, Cross TA and Marshall AG [2005]. Identification of Mycobacterium tuberculosis H37Rv integral membrane proteins by one-dimensional gel electrophoresis and liquid chromatography electrospray ionization tandem mass spectrometry. Proteomics
- Mawuenyega KG et al. [2005]. Mycobacterium tuberculosis functional network analysis by global subcellular protein profiling. Proteomics
- Walters SB et al. [2006]. The Mycobacterium tuberculosis PhoPR two-component system regulates genes essential for virulence and complex lipid biosynthesis. Transcriptome
- Målen H et al. [2010]. Definition of novel cell envelope associated proteins in Triton X-114 extracts of Mycobacterium tuberculosis H37Rv. Proteomics
- Kruh NA et al. [2010]. Portrait of a pathogen: the Mycobacterium tuberculosis proteome in vivo. Proteomics
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
