Gene Rv3485c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Function unknown; supposed to be involved in cellular metabolism. |
| Product | Probable short-chain type dehydrogenase/reductase |
| Comments | Rv3485c, (MTCY13E12.38c), len: 314 aa. Probable short-chain dehydrogenase/reductase, similar, but longer 41 aa, to P71824|Rv0769|MTCY369.14 putative short-chain type dehydrogenase/reductase CY369.14 from Mycobacterium tuberculosis (248 aa), FASTA scores: opt: 462, E(): 1.8e-19, (34.0% identity in 253 aa overlap). Also similar to various dehydrogenases e.g. P25529|HDHA_ECOLI|HSDH|B1619 NAD-dependent 7 alpha-hydroxysteroid dehydrogenase (SDR family) from Escherichia coli strain K12 (alias BAB35750|ECS2327 or AAG56608|HDHA for strain O157:H7) (255 aa), FASTA scores: opt: 462, E(): 1.8e-19, (34.7% identity in 248 aa overlap); Q9FD15|RUBG putative reductase (SDR family) from Streptomyces collinus (249 aa), FASTA scores: opt: 446, E(): 1.5e-18, (36.1% identity in 255 aa overlap); BAB51974|MLL5540 putative dehydrogenase from Rhizobium loti (Mesorhizobium loti) (253 aa), FASTA scores: opt: 442, E(): 2.5e-18, (36.25% identity in 251 aa overlap); Q08632|SDR1_PICAB short-chain type dehydrogenase/reductase (SDR family) from Picea abies (Norway spruce) (Picea excelsa) (271 aa), FASTA scores: opt: 441, E(): 3.1e-18, (32.3% identity in 260 aa overlap); Q9A326|CC3380 2-deoxy-D-gluconate 3-dehydrogenase from Caulobacter crescentus (260 aa), FASTA scores: opt: 436, E(): 5.7e-18, (32.8% identity in 253 aa overlap); Q16698|DECR_HUMAN 2,4-dienoyl-CoA reductase, mitochondrial precursor from Homo sapiens (Human) (335 aa), FASTA scores: opt: 430, E(): 1.5e-17, (30.4% identity in 306 aa overlap); etc. Contains short-chain alcohol dehydrogenase family signature (PS00061). Belongs to the short-chain dehydrogenases/reductases family (SDR). |
| Functional category | Intermediary metabolism and respiration |
| Proteomics | Identified by proteomics at the Max Planck Institute for Infection Biology, Berlin, Germany and the Statens Serum Institute (Denmark) (see citations below). Identified in the membrane fraction of M. tuberculosis H37Rv using 1D-SDS-PAGE and uLC-MS/MS (See Gu et al., 2003). Predicted secreted protein - identified in culture filtrates of M. tuberculosis H37Rv; signal peptide predicted (See Malen et al., 2007). Identified by mass spectrometry in whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate or membrane protein fraction (See de Souza et al., 2011). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Disruption of this gene provides a growth advantage for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3904622 | 3905566 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3485c|Rv3485c
MNSRAPRNLAVSSPSAQVTGRMVQNGENLFQFRREGPQVQLSFQDRTYLVTGGGSGIGKGVAAGLVAAGAAVMIVGRNPDKLAAAVKDIEALKTGAIGYEPADITDEEQTLRVVDAATAWHGRLHGVVHCAGGSQTIGPITQIDSQAWRRTVDLNVNGTMYVLKHAARELVRGGGGSFVGISSIAASNTHRWFGAYGVTKSAVDHMMKLAADELGPSWVRVNSIRPGLIRTDLVVPVTESPELSADYRVCTPLPRVGEVEDVANLAMFLLSDAASWITGQVINVDGGHMLRRGPDFSPMLEPVFGADGLRGVVG
Bibliography
- Jungblut PR, Schaible UE, Mollenkopf HJ, Zimny-Arndt U, Raupach B, Mattow J, Halada P, Lamer S, Hagens K and Kaufmann SH [1999]. Comparative proteome analysis of Mycobacterium tuberculosis and Mycobacterium bovis BCG strains: towards functional genomics of microbial pathogens. Proteomics
- Rosenkrands I et al. [2000]. Towards the proteome of Mycobacterium tuberculosis. Proteomics
- Gu S et al. [2003]. Comprehensive proteomic profiling of the membrane constituents of a Mycobacterium tuberculosis strain. Proteomics
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Målen H et al. [2007]. Comprehensive analysis of exported proteins from Mycobacterium tuberculosis H37Rv. Proteomics
- Gurvitz A et al. [2008]. Function of heterologous Mycobacterium tuberculosis InhA, a type 2 fatty acid synthase enzyme involved in extending C20 fatty acids to C60-to-C90 mycolic acids, during de novo lipoic acid synthesis in Saccharomyces cerevisiae. Function
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
