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virulence, detoxification, adaptation
information pathways
cell wall and cell processes
stable RNAs
insertion seqs and phages
PE/PPE
intermediary metabolism and respiration
unknown
regulatory proteins
conserved hypotheticals
lipid metabolism
pseudogenes
General annotation
TypeCDS
FunctionFunction unknown; probably involved in cellular metabolism. Predicted to be involved in lipid catabolism.
ProductProbable dehydrogenase
CommentsRv3537, (MTCY03C7.19c), len: 563 aa. Probable kstD, dehydrogenase, similar to many dehydrogenases or hypothetical proteins e.g. Q9I1M6|PA2243 hypothetical protein from Pseudomonas aeruginosa (577 aa), FASTA scores: opt: 984, E(): 1.2e-48, (34.75% identity in 573 aa overlap); Q06401|3O1D_COMTE 3-oxosteroid 1-dehydrogenase from Comamonas testosteroni (Pseudomonas testosteroni) (573 aa), FASTA scores: opt: 955, E(): 5.5e-47, (33.05% identity in 590 aa overlap); Q9RA02|KSTD1 3-ketosteroid dehydrogenase from Rhodococcus erythropolis (510 aa), FASTA scores: opt: 631, E(): 1.4e-28, (39.15% identity in 557 aa overlap); P77815|KSDD 3-ketosteroid-1-dehydrogenase from Nocardioides simplex (Arthrobacter simplex) (515 aa), FASTA scores: opt: 469, E(): 2.4e-19, (35.45% identity in 564 aa overlap); etc.
Functional categoryIntermediary metabolism and respiration
ProteomicsIdentified in the cell membrane fraction of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). Identified by mass spectrometry in M. tuberculosis H37Rv-infected guinea pig lungs at 90 days but not 30 days (See Kruh et al., 2010).
MutantNon-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Required for survival in primary murine macrophages, by transposon site hybridization (TraSH) in H37Rv (See Rengarajan et al., 2005). Essential gene for in vitro growth of H37Rv on cholesterol, by sequencing of Himar1-based transposon mutagenesis (See Griffin et al., 2011).
Check for mutants available at TARGET website
Coordinates
TypeStartEndOrientation
CDS39753693977060+
Genomic sequence
Feature type Upstream flanking region (bp) Downstream flanking region (bp) Update
       
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3537|kstD
MTVQEFDVVVVGSGAAGMVAALVAAHRGLSTVVVEKAPHYGGSTARSGGGVWIPNNEVLKRRGVRDTPEAARTYLHGIVGEIVEPERIDAYLDRGPEMLSFVLKHTPLKMCWVPGYSDYYPEAPGGRPGGRSIEPKPFNARKLGADMAGLEPAYGKVPLNVVVMQQDYVRLNQLKRHPRGVLRSMKVGARTMWAKATGKNLVGMGRALIGPLRIGLQRAGVPVELNTAFTDLFVENGVVSGVYVRDSHEAESAEPQLIRARRGVILACGGFEHNEQMRIKYQRAPITTEWTVGASANTGDGILAAEKLGAALDLMDDAWWGPTVPLVGKPWFALSERNSPGSIIVNMSGKRFMNESMPYVEACHHMYGGEHGQGPGPGENIPAWLVFDQRYRDRYIFAGLQPGQRIPSRWLDSGVIVQADTLAELAGKAGLPADELTATVQRFNAFARSGVDEDYHRGESAYDRYYGDPSNKPNPNLGEVGHPPYYGAKMVPGDLGTKGGIRTDVNGRALRDDGSIIDGLYAAGNVSAPVMGHTYPGPGGTIGPAMTFGYLAALHIADQAGKR