Gene Rv3560c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Function unknown, but involved in lipid degradation. |
| Product | Probable acyl-CoA dehydrogenase FadE30 |
| Comments | Rv3560c, (MTCY06G11.07c), len: 385 aa. Probable fadE30, acyl-CoA dehydrogenase, similar to many e.g. Q9I4V2|PA1022 from Pseudomonas aeruginosa (381 aa), FASTA scores: opt: 845, E(): 1.6e-47, (39.2% identity in 388 aa overlap); Q9A5G9|CC2478 from Caulobacter crescentus (407 aa), FASTA scores: opt: 734, E(): 2.8e-40, (35.5% identity in 386 aa overlap); Q9RJX2|SCF37.29c from Streptomyces coelicolor (393 aa), FASTA scores: opt: 656, E(): 3.2e-35, (37.9% identity in 351 aa overlap); etc. Also similar to acyl-CoA dehydrogenases from Mycobacterium tuberculosis e.g. P95280|FADE17|Rv1934c|MTCY09F9.30 (409 aa), FASTA scores: opt: 939, E(): 1.4e-53, (43.8% identity in 404 aa overlap). Could belong to the acyl-CoA dehydrogenases family. |
| Functional category | Lipid metabolism |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Required for growth in C57BL/6J mouse spleen, by transposon site hybridization (TraSH) in H37Rv (See Sassetti and Rubin, 2003). Required for survival in primary murine macrophages, by transposon site hybridization (TraSH) in H37Rv (See Rengarajan et al., 2005). Non-essential gene for in vitro growth of H37Rv, but essential for in vitro growth on cholesterol; by sequencing of Himar1-based transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 4000432 | 4001589 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3560c|fadE30
MQDVEEFRAQVRGWLADNLAGEFAALKGLGGPGREHEAFEERRAWNQRLAAAGLTCLGWPEEHGGRGLSTAHRVAFYEEYARADAPDKVNHFGEELLGPTLIAFGTPQQQRRFLPRIRDVTELWCQGYSEPGAGSDLASVATTAELDGDQWVINGQKVWTSLAHLSQWCFVLARTEKGSQRHAGLSYLLVPLDQPGVQIRPIVQITGTAEFNEVFFDDARTDADLVVGAPGDGWRVAMATLTFERGVSTLGQQIVYARELSNLVELARRTAAADDPLIRERLTRAWTGLRAMRSYALATMEGPAVEQPGQDNVSKLLWANWHRNLGELAMDVIGKPGMTMPDGEFDEWQRLYLFTRADTIYGGSNEIQRNIIAERVLGLPREAKG
Bibliography
- Sassetti CM and Rubin EJ [2003]. Genetic requirements for mycobacterial survival during infection. Mutant
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Rengarajan J et al. [2005]. Genome-wide requirements for Mycobacterium tuberculosis adaptation and survival in macrophages. Mutant
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
