Gene Rv3568c (bphC)
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Catalyzes the extradiol cleavage of 3,4-dihydroxy-9,10-seconandrost-1,3,5(10)-triene-9,17-dione (3,4-DHSA) |
| Product | 3,4-DHSA dioxygenase |
| Comments | Rv3568c, (MTCY06G11.15c), len: 300 aa. HsaC, highly similar to e.g. Q9KWQ5|BPHC5 from Rhodococcus sp. RHA1 (300 aa), FASTA scores: opt: 1715, E(): 3.8e-103, (82.15% identity in 297 aa overlap); O50479|EDOB from Rhodococcus rhodochrous (300 aa) FASTA scores: opt: 1714, E(): 4.4e-103, (82.5% identity in 297 aa overlap); O69359|BPHC6 from Rhodococcus erythropolis (300 aa), FASTA scores: opt: 1647, E(): 9.1e-99, (78.25% identity in 299 aa overlap); Q9RBT2|BPHC1 from Pseudomonas sp. SY5 (301 aa) Pseudomonas sp. SY5 (298 aa) FASTA scores: opt: 767, E(): 3.9e-42, (42.8% identity in 299 aa overlap); P47228|BPHC_BURCE from Burkholderia cepacia (Pseudomonas cepacia) (297 aa), FASTA scores: opt: 670, E(): 6.8e-36, (40.55% identity in 296 aa overlap); etc. Contains PS00082 Extradiol ring-cleavage dioxygenases signature. Belongs to the extradiol ring-cleavage dioxygenase family. |
| Functional category | Intermediary metabolism and respiration |
| Proteomics | Identified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in the culture filtrate, membrane protein fraction, and whole cell lysates of M. tuberculosis H37Rv (See de Souza et al., 2011). |
| Operon | Rv3569c and Rv3568c, Rv3568c and Rv3567c are co-transcribed in M. bovis BCG, by RT-PCR (See Anderton et al., 2006). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non-essential gene for in vitro growth of H37Rv, but essential for in vitro growth on cholesterol; by sequencing of Himar1-based transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 4009297 | 4010199 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3568c|hsaC
MSIRSLGYLRIEATDMAAWREYGLKVLGMVEGKGAPEGALYLRMDDFPARLVVVPGEHDRLLEAGWECANAEGLQEIRNRLDLEGTPYKEATAAELADRRVDEMIRFADPSGNCLEVFHGTALEHRRVVSPYGHRFVTGEQGMGHVVLSTRDDAEALHFYRDVLGFRLRDSMRLPPQMVGRPADGPPAWLRFFGCNPRHHSLAFLPMPTSSGIVHLMVEVEQADDVGLCLDRALRRKVPMSATLGRHVNDLMLSFYMKTPGGFDIEFGCEGRQVDDRDWIARESTAVSLWGHDFTVGARG
Bibliography
- Anderton MC et al. [2006]. Characterization of the putative operon containing arylamine N-acetyltransferase (nat) in Mycobacterium bovis BCG. Operon
- Kendall SL, Withers M, Soffair CN, Moreland NJ, Gurcha S, Sidders B, Frita R, Ten Bokum A, Besra GS, Lott JS and Stoker NG [2007]. A highly conserved transcriptional repressor controls a large regulon involved in lipid degradation in Mycobacterium smegmatis and Mycobacterium tuberculosis. Regulation
- Van der Geize R et al. [2007]. A gene cluster encoding cholesterol catabolism in a soil actinomycete provides insight into Mycobacterium tuberculosis survival in macrophages. Product Function
- Målen H et al. [2010]. Definition of novel cell envelope associated proteins in Triton X-114 extracts of Mycobacterium tuberculosis H37Rv. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
