Gene Rv3603c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Function unknown |
| Product | Conserved hypothetical alanine and leucine rich protein |
| Comments | Rv3603c, (MTCY07H7B.19), len: 303 aa. Conserved hypothetical ala-, leu-rich protein, identical except at N-terminus (really different) to AAK48066|MT3708 chalcone/stilbene synthase family protein from Mycobacterium tuberculosis strain CDC1551 (361 aa) FASTA scores: opt: 1742, E(): 8.3e-95, (100.0% identity in 275 aa overlap). Equivalent to O69525|MLCB2548.02c|ML0229 hypothetical 32.7 KDA protein from Mycobacterium leprae (309 aa), FASTA scores: opt: 947, E(): 2.4e-48, (67.85% identity in 311 aa overlap). Also highly similar to Q9X845|SCE126.02c hypothetical 42.2 KDA protein from Streptomyces coelicolor (420 aa), FASTA scores: opt: 683, E(): 8.5e-33, (49.3% identity in 284 aa overlap). |
| Functional category | Conserved hypotheticals |
| Proteomics | Identified by mass spectrometry in whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate or membrane protein fraction (See de Souza et al., 2011). |
| Transcriptomics | mRNA identified by microarray analysis and down-regulated after 4h, 24h and 96h of starvation (see citation below). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Essential gene domain for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 4045207 | 4046118 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3603c|Rv3603c
MERFDGLRPARLKVGIISAGRVGTALGVALQRADHVVVACSAISHASRRRAQRRLPDTPVLPPLDVAASAELLLLAVTDSELAGLVSGLAATSAVRPQTIVAHTSGANGIGILAPLAQQGCIPLAIHPAMTFTGSDEDISRLPDTCFGITAADDVGYAIGQSLVLEMGGEPFCVREDARILYHAALAHASNHIVTVLADALEALRAALSGGELLGQQTVDDQPGGIVERIVGPLARAALENTLQRGQAALTGPVARGDAAAVADHLAALADVDAALAQAYRINALRTAQRAHAPADVVEVLTA
Bibliography
- Betts JC et al. [2002]. Evaluation of a nutrient starvation model of Mycobacterium tuberculosis persistence by gene and protein expression profiling. Transcriptome
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
