Gene Rv3618
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Function unknown |
| Product | Possible monooxygenase |
| Comments | Rv3618, (MTCY15C10.34c, MTCY07H7B.04c), len: 395 aa. Possible monooxygenase, similar to others (principally bacterial luciferases alpha chain) e.g. Q9JN87|MMYO putative alkanal monooxygenase from Streptomyces coelicolor (373 aa), FASTA scores: opt: 949, E(): 8.9e-54, (41.7% identity in 374 aa overlap); Q9EUT9|limb limonene monooxygenase from Rhodococcus erythropolis (387 aa), FASTA scores: opt: 856, E(): 9.1e-48, (42.0% identity in 388 aa overlap); AAK72698 LUXA-like protein from Bradyrhizobium japonicum (458 aa) FASTA scores: opt: 350, E(): 4.4e-15, (29.7% identity in 347 aa overlap); Q9K4C1|2SC6G5.34c putative alkanal monooxygenase (luciferase) from Streptomyces coelicolor (342 aa), FASTA scores: opt: 291, E(): 2.2e-11, (26.5% identity in 362 aa overlap); etc. Also similar to P95278|Rv1936|MTCY09F9.28c hypothetical 41.8 KDA protein from Mycobacterium tuberculosis (369 aa), FASTA scores: opt: 473, E(): 4.3e-23, (32.55% identity in 378 aa overlap). |
| Functional category | Intermediary metabolism and respiration |
| Proteomics | Identified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in the membrane protein fraction and whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate (See de Souza et al., 2011). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in CDC1551 strain (see Lamichhane et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Found to be deleted (partially or completely) in one or more clinical isolates (See Tsolaki et al., 2004). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 4058698 | 4059885 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3618|Rv3618
MKAPLRFGVFITPFHPTGQSPTVALQYDMERVVALDRLGYDEAWFGEHHSGGYELIACPEVFIAAAAERTTHIRLGTGVVSLPYHHPLMVADRWVLLDHLTRGRVMFGTGPGALPSDAYMMGIDPVEQRRMMQESLEAILALFRAAPDERIDRHSDWFTLREAQLHIRPYTWPYPEIATAAMISPSGPRLAGALGTSLLSLSMSVPGGYAALETAWGVVREQAAKAGRGEPDRADWRVLSIMHLSDSRDQAIDDCTYGLPDFSRYFGAAGFVPLANTVEGTQSSREFVEQYAAKGNCCIGTPDDAIAHIEDLLHRSGGFGTLLLLGHDWAPPPATFHSYELFARAVIPYFKGQLAAPRASHEWARGKRDQLIGRAGEAVVKAITEHVAEQGEAGS
Bibliography
- Lamichhane G et al. [2003]. A postgenomic method for predicting essential genes at subsaturation levels of mutagenesis: application to Mycobacterium tuberculosis. Mutant
- Tsolaki AG, Hirsh AE, DeRiemer K, Enciso JA, Wong MZ, Hannan M, Goguet de la Salmoniere YO, Aman K, Kato-Maeda M and Small PM [2004]. Functional and evolutionary genomics of Mycobacterium tuberculosis: insights from genomic deletions in 100 strains. Mutant
- Målen H et al. [2010]. Definition of novel cell envelope associated proteins in Triton X-114 extracts of Mycobacterium tuberculosis H37Rv. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
