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virulence, detoxification, adaptation
information pathways
cell wall and cell processes
stable RNAs
insertion seqs and phages
PE/PPE
intermediary metabolism and respiration
unknown
regulatory proteins
conserved hypotheticals
lipid metabolism
pseudogenes
General annotation
TypeCDS
FunctionFunction unknown
ProductPossible monooxygenase
CommentsRv3618, (MTCY15C10.34c, MTCY07H7B.04c), len: 395 aa. Possible monooxygenase, similar to others (principally bacterial luciferases alpha chain) e.g. Q9JN87|MMYO putative alkanal monooxygenase from Streptomyces coelicolor (373 aa), FASTA scores: opt: 949, E(): 8.9e-54, (41.7% identity in 374 aa overlap); Q9EUT9|limb limonene monooxygenase from Rhodococcus erythropolis (387 aa), FASTA scores: opt: 856, E(): 9.1e-48, (42.0% identity in 388 aa overlap); AAK72698 LUXA-like protein from Bradyrhizobium japonicum (458 aa) FASTA scores: opt: 350, E(): 4.4e-15, (29.7% identity in 347 aa overlap); Q9K4C1|2SC6G5.34c putative alkanal monooxygenase (luciferase) from Streptomyces coelicolor (342 aa), FASTA scores: opt: 291, E(): 2.2e-11, (26.5% identity in 362 aa overlap); etc. Also similar to P95278|Rv1936|MTCY09F9.28c hypothetical 41.8 KDA protein from Mycobacterium tuberculosis (369 aa), FASTA scores: opt: 473, E(): 4.3e-23, (32.55% identity in 378 aa overlap).
Functional categoryIntermediary metabolism and respiration
ProteomicsIdentified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in the membrane protein fraction and whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate (See de Souza et al., 2011).
MutantNon-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in CDC1551 strain (see Lamichhane et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Found to be deleted (partially or completely) in one or more clinical isolates (See Tsolaki et al., 2004).
Check for mutants available at TARGET website
Coordinates
TypeStartEndOrientation
CDS40586984059885+
Genomic sequence
Feature type Upstream flanking region (bp) Downstream flanking region (bp) Update
       
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3618|Rv3618
MKAPLRFGVFITPFHPTGQSPTVALQYDMERVVALDRLGYDEAWFGEHHSGGYELIACPEVFIAAAAERTTHIRLGTGVVSLPYHHPLMVADRWVLLDHLTRGRVMFGTGPGALPSDAYMMGIDPVEQRRMMQESLEAILALFRAAPDERIDRHSDWFTLREAQLHIRPYTWPYPEIATAAMISPSGPRLAGALGTSLLSLSMSVPGGYAALETAWGVVREQAAKAGRGEPDRADWRVLSIMHLSDSRDQAIDDCTYGLPDFSRYFGAAGFVPLANTVEGTQSSREFVEQYAAKGNCCIGTPDDAIAHIEDLLHRSGGFGTLLLLGHDWAPPPATFHSYELFARAVIPYFKGQLAAPRASHEWARGKRDQLIGRAGEAVVKAITEHVAEQGEAGS