Gene Rv3671c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Function unknown; hydrolyses of peptides and/or proteins (possibly cleaved preferentially after serine residue). |
| Product | Membrane-associated serine protease |
| Comments | Rv3671c, (MTV025.019c), len: 397 aa. Serine protease membrane protein, equivalent to Q9CB95|ML2298 putative membrane-associated serine protease from Mycobacterium leprae (401 aa), FASTA scores: opt: 2061, E(): 2.3e-108, (80.9% identity in 398 aa overlap). Also similar to many serine proteases, but generally with extended N-terminus, e.g. Q9X932|SCH5.30c putative serine protease (fragment) from Streptomyces coelicolor (385 aa), FASTA scores: opt: 835, E(): 1.2e-39, (39.9% identity in 386 aa overlap); Q9Z6T0|DEGP_CHLPN|HTRA|CPN0979|CP0877 probable serine protease do-like precursor from Chlamydia pneumoniae (Chlamydophila pneumoniae) (488 aa), FASTA scores: opt: 285, E(): 1e-08, (29.05% identity in 296 aa overlap); P73354|HTRA|SLR1204 serine protease from Synechocystis sp. strain PCC 6803 (452 aa), FASTA scores: opt: 284, E(): 1.1e-08, (29.55% identity in 308 aa overlap); Q9RWC4|DR0745 periplasmic serine protease, HTRA/DEGQ/DEGS family from Deinococcus radiodurans (366 aa), FASTA scores: opt: 271, E(): 4.9e-08, (35.45% identity in 206 aa overlap); etc. Also similar, but longer 114 aa at the N-terminus, to Q9S2P8|SC5F7.13 putative peptidase from Streptomyces coelicolor (282 aa), FASTA scores: opt: 594, E(): 3.1e-26, (38.95% identity in 285 aa overlap). And similar, but longer 146 aa at the N-terminus, to O07175|PEPA|Rv0125|MTCI418B.07 from Mycobacterium tuberculosis (355 aa), FASTA scores: opt: 295, E(): 2.2e-09, (29.55% identity in 254 aa overlap); and Q9CCY9|ML2659 probable secreted serine protease from Mycobacterium leprae FASTA scores: opt: 286, E(): 6.9e-09, (30.6% identity in 255 aa overlap). Contains PS00135 Serine proteases, trypsin family, serine active site. Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007). |
| Functional category | Intermediary metabolism and respiration |
| Proteomics | Identified in the membrane fraction of M. tuberculosis H37Rv using nanoLC-MS/MS; predicted integral membrane protein (See Xiong et al., 2005). Predicted transmembrane protein - identified in culture filtrates of M. tuberculosis H37Rv; signal peptide predicted (See Malen et al., 2007). Identified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in the culture filtrate, membrane protein fraction, and whole cell lysates of M. tuberculosis H37Rv (See de Souza et al., 2011). |
| Mutant | Essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). M. tuberculosis H37Rv transposon mutant is sensitive to pH 4.5; mutant is able to control intrabacterial pH in bone marrow derived macrophages but not in IFN-gamma-activated macrophages; growth of mutant is impaired in C57BL/6 mice (See Vandal et al., 2008). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 4112322 | 4113515 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3671c|Rv3671c
MTPSQWLDIAVLAVAFIAAISGWRAGALGSMLSFGGVLLGATAGVLLAPHIVSQISAPRAKLFAALFLILALVVVGEVAGVVLGRAVRGAIRNRPIRLIDSVIGVGVQLVVVLTAAWLLAMPLTQSKEQPELAAAVKGSRVLARVNEAAPTWLKTVPKRLSALLNTSGLPAVLEPFSRTPVIPVASPDPALVNNPVVAATEPSVVKIRSLAPRCQKVLEGTGFVISPDRVMTNAHVVAGSNNVTVYAGDKPFEATVVSYDPSVDVAILAVPHLPPPPLVFAAEPAKTGADVVVLGYPGGGNFTATPARIREAIRLSGPDIYGDPEPVTRDVYTIRADVEQGDSGGPLIDLNGQVLGVVFGAAIDDAETGFVLTAGEVAGQLAKIGATQPVGTGACVS
Bibliography
- Xiong Y, Chalmers MJ, Gao FP, Cross TA and Marshall AG [2005]. Identification of Mycobacterium tuberculosis H37Rv integral membrane proteins by one-dimensional gel electrophoresis and liquid chromatography electrospray ionization tandem mass spectrometry. Proteomics
- Ribeiro-Guimarães ML et al. [2007]. Comparative genomics of mycobacterial proteases. Homology
- Målen H et al. [2007]. Comprehensive analysis of exported proteins from Mycobacterium tuberculosis H37Rv. Proteomics
- Vandal OH et al. [2008]. A membrane protein preserves intrabacterial pH in intraphagosomal Mycobacterium tuberculosis. Mutant
- Vandal OH et al. [2009]. Acid-susceptible mutants of Mycobacterium tuberculosis share hypersusceptibility to cell wall and oxidative stress and to the host environment. Mutant
- Målen H et al. [2010]. Definition of novel cell envelope associated proteins in Triton X-114 extracts of Mycobacterium tuberculosis H37Rv. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
