Gene Rv3683
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Function unknown |
| Product | Conserved protein |
| Comments | Rv3683, (MTV025.031), len: 319 aa. Conserved protein, equivalent to Q9CB84|ML2309 hypothetical protein from Mycobacterium leprae (330 aa) FASTA scores: opt: 1791, E(): 9e-107, (85.45% identity in 296 aa overlap). Also similar to Q9X935|SCH66.03 conserved hypothetical protein from Streptomyces coelicolor (309 aa) FASTA scores: opt: 610, E(): 1.4e-31, (51.45% identity in 307 aa overlap); and Q9RRY7|YN45_DEIRA|DR2345 hypothetical protein from Deinococcus radiodurans (305 aa) FASTA scores: opt: 243, E(): 3.2e-08, (31.1% identity in 315 aa overlap) and some similarity to other hypothetical bacterial proteins e.g. Q9CF81|YQED from Lactococcus lactis (subsp. lactis) (Streptococcus lactis) (278 aa) FASTA scores: opt: 200, E(): 1.6e-05, (26.85% identity in 287 aa overlap). Predicted to be an outer membrane protein (See Song et al., 2008). |
| Functional category | Conserved hypotheticals |
| Proteomics | Identified by proteomics (See Rosenkrands et al., 2000). Identified in the membrane fraction of M. tuberculosis H37Rv using 1D-SDS-PAGE and uLC-MS/MS; predicted transmembrane protein (See Gu et al., 2003). Identified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in the membrane protein fraction and whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate (See de Souza et al., 2011). Translational start site supported by proteomics data (See de Souza et al., 2011) (See Kelkar et al., 2011). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Required for growth in C57BL/6J mouse spleen, by transposon site hybridization (TraSH) in H37Rv (See Sassetti and Rubin, 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 4124417 | 4125376 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3683|Rv3683
MAAVLPTLIRTGAVALGSAIAGIGYAALVERNAFVLREVTMPVLTPGSTPLRVLHISDLHMLPNQHRKQAWLRELASWEPDLVVNTGDNLAHPKAVPAVVQTLSDLLSRPGVFVFGSNDYFGPRLKNPMNYLTSPDHRVRGAALPWQDLRAAFTERGWLDLTHTRREFEVAGLHIAAAGVDDPHIDRDRYDTIAGPASPAANLRLGLTHSPEPRVLDRFAADGYQLVLAGHTHGGQLCLPLYGALVTNCGLDRSRAKGASHWGANMRLHVSAGIGTSPFAPVRFCCRPEATLLTLIATPMGGRDSSSNLGRSQPTVSVR
Bibliography
- Rosenkrands I et al. [2000]. Towards the proteome of Mycobacterium tuberculosis. Proteomics
- Sassetti CM and Rubin EJ [2003]. Genetic requirements for mycobacterial survival during infection. Mutant
- Gu S et al. [2003]. Comprehensive proteomic profiling of the membrane constituents of a Mycobacterium tuberculosis strain. Proteomics
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Song H, Sandie R, Wang Y, Andrade-Navarro MA and Niederweis M [2008]. Identification of outer membrane proteins of Mycobacterium tuberculosis. Localization
- Målen H et al. [2010]. Definition of novel cell envelope associated proteins in Triton X-114 extracts of Mycobacterium tuberculosis H37Rv. Proteomics
- Kelkar DS et al. [2011]. Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry. Proteomics Sequence
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- de Souza GA et al. [2011]. Proteogenomic analysis of polymorphisms and gene annotation divergences in prokaryotes using a clustered mass spectrometry-friendly database. Proteomics Sequence
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- Mazandu GK et al. [2012]. Function prediction and analysis of mycobacterium tuberculosis hypothetical proteins. Function
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
