Gene Rv3693
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Unknown |
| Product | Possible conserved membrane protein |
| Comments | Rv3693, (MTV025.041), len: 440 aa (alternative start at 41910). Possible conserved membrane protein, similar to Q9KYW2|SCE33.21 putative lipoprotein from Streptomyces coelicolor (436 aa), FASTA scores: opt: 875, E(): 3.3e-46, (56.25% identity in 448 aa overlap); Q9AAN0|CC0567 hypothetical protein from Caulobacter crescentus (437 aa), FASTA scores: opt: 355, E(): 2.3e-14, (30.9% identity in 450 aa overlap); P73233|SLR2013 hypothetical 48.5 KDA protein from Synechocystis sp. strain PCC 6803 (435 aa), FASTA scores: opt: 340, E(): 1.9e-13, (29.7% identity in 438 aa overlap); etc. Equivalent to AAK48162 from Mycobacterium tuberculosis strain CDC1551 (475 aa) but shorter 35 aa. Also similar to other hypothetical proteins from Mycobacterium tuberculosis; MTV014_7; MTV007_27; and MTCY71_36 M. Predicted to be an outer membrane protein (See Song et al., 2008). |
| Functional category | Cell wall and cell processes |
| Proteomics | Predicted secreted protein - identified in culture filtrates of M. tuberculosis H37Rv; signal peptide predicted (See Malen et al., 2007). Identified in the cytosol and cell membrane fraction of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). Identified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in the membrane protein fraction of M. tuberculosis H37Rv but not the culture filtrate or membrane protein fraction (See de Souza et al., 2011). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 4134726 | 4136048 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3693|Rv3693
MILTGRTGLLALICVLPIALSPWPARAFVMLLVALAVAVTVDTLLAASTRKLRFTRSPYTSARLGQPVDASLLLCNGGRRRFRGQVRDAWPPSARAQPHTHDVDVAAGQRQQVHTALRPVRRGDQRAAMVTARSIGPLGLAGRQSSQSVPGLVRVLPPFLSRKHLPSRLAKLREIDGLLPTLIRGQGTEFDSLREYVVGDDVRSIDWRASARRADVMVRTWRPERDRRVVIVLDTGRMAAGRVGVDPTAADPAGWPRLDWSMDAALLLAALASRAGDHVDFLAHDRISRAGVFGASRSELLAQLVDAMAPLRPALIESDWHAMIATILRRTRRRSLVVLLTDLNATALDEGLLPVLPQLSARHHVLVAAVADPRVDQLAAGRSDAAAVYDAAAAERARNDRRAIASQLRRGGVDVIDAPPAEIAPGLADRYLAMKATGRL
Bibliography
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Mawuenyega KG et al. [2005]. Mycobacterium tuberculosis functional network analysis by global subcellular protein profiling. Proteomics
- Målen H et al. [2007]. Comprehensive analysis of exported proteins from Mycobacterium tuberculosis H37Rv. Proteomics
- Song H, Sandie R, Wang Y, Andrade-Navarro MA and Niederweis M [2008]. Identification of outer membrane proteins of Mycobacterium tuberculosis. Localization
- Målen H et al. [2010]. Definition of novel cell envelope associated proteins in Triton X-114 extracts of Mycobacterium tuberculosis H37Rv. Proteomics
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
