Gene Rv3714c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Function unknown |
| Product | Conserved hypothetical protein |
| Comments | Rv3714c, (MTV025.062c), len: 296 aa. Conserved hypothetical protein, highly similar to O07396|MAV346 MAV346 protein from Mycobacterium avium (346 aa) FASTA scores: opt: 834, E(): 2.2e-46, (50.0% identity in 286 aa overlap); and also highly similar to several proteins from Mycobacterium tuberculosis e.g. O53421|Rv1073|MTV017.26 (283 aa), FASTA scores: opt: 869, E(): 1e-48, (51.1% identity in 270 aa overlap); P71763|Rv1482c|MTCY277.03c (339 aa), FASTA scores: opt: 775, E(): 1.3e-42, (46.35% identity in 289 aa overlap); P96837|Rv3555c|MTCY06G11.02c (289 aa), FASTA scores: opt: 733, E(): 5.9e-40, (44.15% identity in 281 aa overlap); etc. Partially similar to Q9Z512|UVRC_STRCO|SCC54.13c excinuclease ABC subunit C from Streptomyces coelicolor (728 aa), FASTA scores: opt: 122, E(): 2.5, (27.0% identity in 174 aa overlap). Equivalent to AAK48186 from Mycobacterium tuberculosis strain CDC1551 (341 aa) but shorter 45 aa. |
| Functional category | Conserved hypotheticals |
| Proteomics | Identified in the cell membrane fraction of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv and CDC1551 strains (see Sassetti et al., 2003 and Lamichhane et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 4158931 | 4159821 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3714c|Rv3714c
MLISRMSVRSASMSVMGDVFIGSEAITAGRLTRHELQRWYQPMFRGVYVSRRSVPTLWDRTVGAWLATRRHGVIAGNAASALHGAQWVDVDVAIELISPTTRPQHGLVIRRETLCDDEITRVVGLPVTTLARTAYDLGRHLSRGEAVARLDALMRATPFSRDDVLLLAKRHAGARGVRRLRDVLPLVDGGAASPKETWLRLLLIDAGLPVPTTQIPVVHRWRNVGVLDMGWEKYMVAAEYDGDQHRSDRGRYVKDQRRLRKLAELGWIVIRVIAEDNPDDVVNRVRAALLARGWRP
Bibliography
- Lamichhane G et al. [2003]. A postgenomic method for predicting essential genes at subsaturation levels of mutagenesis: application to Mycobacterium tuberculosis. Mutant
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Mawuenyega KG et al. [2005]. Mycobacterium tuberculosis functional network analysis by global subcellular protein profiling. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
