Gene Rv3728
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Unknown, but seems involved in efflux system (probably sugar or drug transport). |
| Product | Probable conserved two-domain membrane protein |
| Comments | Rv3728, (MTV025.076), len: 1065 aa. Probable conserved transmembrane protein organised into two domains. Domain comprising the first ~510 aa residues is similar to various multidrug resistance and efflux proteins and contains sugar transport protein signature 1 (PS00216). Domain corresponding to the last 550 aa residues contains cyclic nucleotide-binding domain signature 2 (PS00889) and is very similar to Q50733|YP65_MYCTU|Rv2565|MT2641|MTCY9C4.03c hypothetical 62.1 kDa protein from Mycobacterium tuberculosis (31.0% identity in 546 aa overlap). Highly similar to O05884|Rv3239c|MTCY20B11.14c probable transmembrane transport protein from Mycobacterium tuberculosis (1048 aa) FASTA scores: opt: 4328, E(): 5e-201, (64.15% identity in 1046 aa overlap). N-terminal end similar to P71879|Rv2333c|MTCY3G12.01|MTCY98.02c (537 aa); P71836|Rv0783c|MTCY369.27c (540 aa); and O07753|Rv1877|MTCY180.41c (687 aa). Seems belong to the sugar transporter family. Possibly member of major facilitator superfamily (MFS). |
| Functional category | Cell wall and cell processes |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 4174873 | 4178070 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3728|Rv3728
VHTVATNNAAPVIAAGPVGPSRRRRRVHAPLTRRRQPSSSAVLLVAAFGAFLAFLDSTIVNVAFPDIQRHFHSDISDLSWMLNAYNIVFAAFLVAAGRLADLMGRKRVFILGVALFTVASGLCAIAESVGELVAFRVLQGIGAAVLVPASLGLVVEAFPAERRAHGVNLWGAAGAIAAGLGPPIGGALIEADGWRWVFLVNLPLGVFAVLAARRALVENRAAGRRRVPDVRGAVLLAFALGLLTLGLIKGPDWGWASLPTSGSLLAAAVAMVGFVMSSRHHPAPMVEPTLLRIQSFVAGTGLTAVASAGFYAYLLTHVLFLNYVWGYTLLEAGMAVAPAALVAAVVAAVLGRVADRHGYRFIVGIGALIWAASLLWYLKVVGSQPDFLGEWLPGQILQGIGVGATFPLLGSAALARLAKGGSYATASAVTGTIRQVGAVIGVAVLVILVGTPAPGAAEEALRHGWALAAICFVAVGIGALSLGRIRPVPAAVEPPPGPPVAPLGARRPPRPAPVASPAAAVAPTPKTSREVNLLEALRFARPDTQQIELQAGSYLFHAGDVSDALYVVRSGRLQVLAGDGAKDEVVAELGRGQVVGELGVLLDAPRSASVRAVRDSSLMRVTKAEFAKIADAGVLGALAGVLAKRQHQTRVASQRTTPEVVVAVVGVDANAPVAMVATELCRALSTRLRAVAPGRVDCDGLERAEQTADRVVLHAAVGDARWREFCLRVADRVVLVASNPAVPVAPLPTRATGADLVLAGRPAGREHRRAWEQLITPRSMHVVRREFVADDLRVLATRIAGRSVGLVLSGGAARACAHLGVLEELEAAGVTVDRFAGTSMGAIIAALAASGLDAAGVDAQIYEHFVRKSHGDYTLPSKGLIRGKRTQSTLRTIFGDHLVEELPKHFRCVSVDLLARRPVVHRQGPLADVVGCSMRLPFLYAPLPYGGTLHVDGGVLDNVPVTTLVGKDGPLIAVNVASGGNPSPASGGHRRGKPRVPGLTDTLLRTMTISSAMASEKVLAQADLVIKPNPIGVGLMEYHQIDRAREAGRIAAREALPQIMELVHG
Bibliography
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
