Gene Rv3729
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Function unknown; probably involved in cellular metabolism. |
| Product | Possible transferase |
| Comments | Rv3729, (MTV025.077), len: 776 aa. Conserved hypothetical protein, possible transferase, similar to several hypothetical proteins and various transferases e.g. O26919|MTH831 molybdenum cofactor biosynthesis MOAA homolog from Methanobacterium thermoautotrophicum (497 aa), FASTA scores: opt: 697, E(): 4.8e-34, (30.7% identity in 492 aa overlap); Q58036|Y619_METJA|MJ0619 hypothetical protein from Methanococcus jannaschii (506 aa), FASTA scores: opt: 670, E(): 2e-32, (30.6% identity in 497 aa overlap); O27968|AF2316 conserved hypothetical protein from Archaeoglobus fulgidus (518 aa), FASTA scores: opt: 477, E(): 6.4e-21, (29.4% identity in 500 aa overlap); BAB60102|TVG0985801 molybdenum cofactor biosynthesis protein from Thermoplasma volcanium (606 aa), FASTA scores: opt: 402, E(): 2.1e-16, (28.1% identity in 509 aa overlap); etc. C-terminus similar to methyltransferases e.g. Q9S0N6|AVED C5-O-methyltransferase from Streptomyces avermitilis (283 aa), FASTA scores: opt: 298, E(): 1.9e-10, (31.5% identity in 292 aa overlap). Also similar to the Mycobacterium tuberculosis proteins P71673|YE05_MYCTU|Rv1405c|MT1449|MTCY21B4.22c (274 aa); and Q50584|Rv1523|MTCY19G5.05c. |
| Functional category | Intermediary metabolism and respiration |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv and CDC1551 strains (see Sassetti et al., 2003 and Lamichhane et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 4178285 | 4180615 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3729|Rv3729
MFVEYTKSICPVCKVVVDAQVNIRHDKVYLRKRCREHGSFEALVYGDAQMYLESARFNKPGTFPLRFQTEVRDGCPSDCGLCPDHKQHACLGLIEVNTHCNLDCPICFADSGHQPDGYAITAAQCERMLDTLVAAEGEPEVVMFSGGEPTIHKQLLEFVDAAQARPVKTVIINTNGIRLASDRRFVDQLATRNRPGHPVHIYLQFDGLDEATHRRIRGHDLRDVKQRALDNCAAAGLTVSLVAAVERGLNEHELGAVIRHGMAQPGVQPVVFQPVTHAGRHVQFDPLTRLTNSDIIACITAQLPEWFRPGDFFPVPCCFPSCRSITYLLTDGEHVVPIPRLLNVEDYLDYVSNRVIPDLAIREALENLWSASAVPGTDTMTAQLQRATAALNCAEGCGINLPEALTHLTDRVFAIVIQDFQDPYTLNVKQLMKCCVQQITPDGRLIPFCAYNSVGYREQVREQLTGVPVPDIVPNAIPLAGLLADAPHGSKQANTGGSIARLAGPTRGAPMALPPQQIKACCADAYSRDIVALLLGDSFHPGGATLTRRLADQLGLRSTGDPRRVADIAAGPGASARLLASDYGVAVDGVDISEINVKRAQAAVAQTGLTERVRFHLGDAESVPLPDDTFDALVCECAFCTFPDKNAAAQQFARILRPGGLAGITDVTVGDGGLPAELTPLAAWVACIADARTVTDYTDILEGAGLRTRHIESHDESLLDMIDRIDARITALHVAAPEILADNGIRHDSVRDFTALARAAVQTGRIGYTLMIAEKP
Bibliography
- Lamichhane G et al. [2003]. A postgenomic method for predicting essential genes at subsaturation levels of mutagenesis: application to Mycobacterium tuberculosis. Mutant
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
