Gene Rv3743c (nmtA)
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Cation-transporting ATPase; possibly catalyzes the transport of a undetermined cation (possibly cadmium) with hydrolyse of ATP [catalytic activity: ATP + H(2)O + undetermined cation(in) = ADP + phosphate + undetermined cation(out)]. |
| Product | Probable cation transporter P-type ATPase CtpJ |
| Comments | Rv3743c, (MTV025.091c), len: 660 aa. Probable ctpJ, cation-transporting P-type ATPase, transmembrane protein highly similar to others e.g. Q9ZBF3|SC9B5.27 putative cation-transporting ATPase from Streptomyces coelicolor (638 aa), FASTA scores: opt: 1635, E(): 2.5e-86, (62.25% identity in 63.95 aa overlap); Q59997|CADA|SLR0797 cadmium-transporting ATPase from Synechocystis sp. strain PCC 6803 (642 aa), FASTA scores: opt: 1474, E(): 4.3e-77, (42.4% identity in 604 aa overlap); P30336|CADA_BACFI probable cadmium-transporting ATPase from Bacillus firmus (723 aa), FASTA scores: opt: 1327, E(): 1.3e-68, (36.6% identity in 626 aa overlap); etc. Also highly similar to O53160|CTPD_MYCTU|Rv1469|MT1515|MTV007.16 probable cation-transporting P-type ATPase D from Mycobacterium tuberculosis (657 aa), FASTA scores: opt: 1845, E(): 2.3e-98, (55.85% identity in 650 aa overlap). Contains PS00154 E1-E2 ATPases phosphorylation site and PS01229 Hypothetical family signature 2. Belongs to the cation transport ATPases family (E1-E2 ATPases). Transcription is repressed by NmtR (See Cavet et al., 2002). |
| Functional category | Cell wall and cell processes |
| Proteomics | Translational start site supported by proteomics data (See Kelkar et al., 2011). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv and CDC1551 strains (see Sassetti et al., 2003 and Lamichhane et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 4193391 | 4195373 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3743c|ctpJ
VAVRELSPARCTSASPLVLARRTKLFALSEMRWAALALGLFSAGLLTQLCGAPQWVRWALFLACYATGGWEPGLAGLQALQRRTLDVDLLMVVAAIGAAAIGQIAEGALLIVIFATSGALEALVTARTADSVRGLMGLAPGTATRVGAGGGEETVNAADLRIGDIVLVRPGERISADATVLAGGSEVDQATVTGEPLPVDKSIGDQVFAGTVNGTGALRIRVDRLARDSVVARIATLVEQASQTKARTQLFIEKVEQRYSIGMVAVTLAVFAVPPLWGETLQRALLRAMTFMIVASPCAVVLATMPPLLAAIANAGRHGVLAKSAIVMEQLGTTTRIAFDKTGTLTRGTPELAGIWVYERRFTDDELLRLAAAAEYPSEHPLGAAIVKAAQSRRIRLPTVGEFTAHPGCRVTARVDGHVIAVGSATALLGTAGAAALEASMITAVDFLQGEGYTVVVVVCDSHPVGLLAITDQLRPEAAAAISAATKLTGAKPVLLTGDNRATADRLGVQVGIDDVRAGLLPDDKVAAVRQLQAGGARLTVVGDGINDAPALAAAHVGIAMGSARSELTLQTADAVVVRDDLTTIPTVIAMSRRARRIVVANLIVAVTFIAGLVVWDLAFTLPLPLGVARHEGSTIIVGLNGLRLLRHTAWRRAAGTAHR
Bibliography
- Cavet JS et al. [2002]. A nickel-cobalt-sensing ArsR-SmtB family repressor. Contributions of cytosol and effector binding sites to metal selectivity. Regulation
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Lamichhane G et al. [2003]. A postgenomic method for predicting essential genes at subsaturation levels of mutagenesis: application to Mycobacterium tuberculosis. Mutant
- Kelkar DS et al. [2011]. Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry. Proteomics Sequence
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
