Gene Rv3797
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Function unknown, but involved in lipid degradation. |
| Product | Probable acyl-CoA dehydrogenase FadE35 |
| Comments | Rv3797, (MTV026.02), len: 593 aa. Probable fadE35, acyl-CoA dehydrogenase, similar to many e.g. Q9HY33|PA3593 from Pseudomonas aeruginosa (575 aa) FASTA scores: opt: 838, E(): 2.1e-46, (35.3% identity in 569 aa overlap); Q9ANZ8|AIDB from Burkholderia pseudomallei (Pseudomonas pseudomallei) (554 aa), FASTA scores: opt: 633, E(): 3.4e-33, (33.1% identity in 480 aa overlap); Q9HX44|PA3972 from Pseudomonas aeruginosa (549 aa) FASTA scores: opt: 560, E(): 1.7e-28, (29.9% identity in 569 aa overlap); P33224|AIDB_ECOLI|B4187 from Escherichia coli strain K12 (541 aa), FASTA scores: opt: 455, E(): 1e-21, (31.15% identity in 514 aa overlap); etc. Also similar to O86368|FADE8|Rv0672|MTCI376.02c acyl-CoA dehydrogenase from Mycobacterium tuberculosis (542 aa), FASTA scores: opt: 479, E(): 2.9e-23, (32.2% identity in 460 aa overlap). Could belong to the acyl-CoA dehydrogenases family. |
| Functional category | Lipid metabolism |
| Proteomics | Identified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in the culture filtrate, membrane protein fraction, and whole cell lysates of M. tuberculosis H37Rv (See de Souza et al., 2011). Translational start site supported by proteomics data (See Kelkar et al., 2011). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 4251085 | 4252866 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3797|fadE35
MPEYDLEAVDKLPFSTPEKAQRYQTENYRGAMGLNWYLTDPTLQFIMAYYLRPDELAFAEPHLTRIGELTGGPVTRWAEETDRNPPRLERYDRWGHDISRVVLPESFIQSKRAVIEARQAVRDDAARAGVKPSLALFAADYLLNQADIGMACALATGGNMVRSLVTAYAPPDVREFVLGKLNSGEWDGEAAQLLTERAGGSDLGALETTATRSGDVWLLNGFKWFASNCAGEAFVVLAKPEGAPDSTRGVATFLVLRTRRDGSRNGVRIRRLKDKLGTRSVASGEIEFVDAEAFLLSGEPSADAGPSDGKGLTRMMELTNRLRLGTASFALGNARRALVESLCYAGQRRAFGGALIDKPLMRRKLAEMVVDVEAALAMVFDGFGAANHRQPRCLPQRIAVPVTKLKTCRLGITVASDAIEIHGGNGYIETWPVARLLRDAQVNTIWEGPDNILCLDVRRGIEQTRAHETLLARLRDAVSVSDDDDTTRLVSRRIEDLDAAITAWTKLDRQLAEARLFPLAQFMGDVYAGALLTEQAAWERATRGTDRKALVARLYARRYLADQGPLRGIDADCDEALQRFDELVAGAFTAEQT
Bibliography
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Målen H et al. [2010]. Definition of novel cell envelope associated proteins in Triton X-114 extracts of Mycobacterium tuberculosis H37Rv. Proteomics
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- Kelkar DS et al. [2011]. Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry. Proteomics Sequence
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
