Gene Rv3809c (ceoA)
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Involved in lipopolysaccharide biosynthesis, in the conversion of UDP-galactopyranose into UDP-galactofuranose through a 2-keto intermediate [catalytic activity: UDP-D-galactopyranose = UDP-D-galacto-1,4-furanose]. |
| Product | UDP-galactopyranose mutase Glf (UDP-GALP mutase) (NAD+-flavin adenine dinucleotide-requiring enzyme) |
| Comments | Rv3809c, (MTV026.14), len: 399 aa. Glf (alternate gene name: ceoA), UDP-galactopyranose mutase (see citations below), identical to previously sequenced gene, and equivalent to Q9CDB8|GLF|ML0092 putative UDP-galactopyranose mutase from Mycobacterium leprae (413 aa), FASTA scores: opt: 2347, E(): 1.3e-140, (86.6% identity in 396 aa overlap). Also highly similar to others e.g. AAK61905|EPSJ UDP-galactopyranose mutase (protein involved in exopolysaccharides biosynthesis) from Streptococcus thermophilus (365 aa), FASTA scores: opt: 972, E(): 5.9e-54, (45.85% identity in 375 aa overlap); P37747|GLF_ECOLI|B2036 UDP-galactopyranose mutase from Escherichia coli strain K12 (367 aa), FASTA scores: opt: 958, E(): 4.5e-53, (43.55% identity in 379 aa overlap); O86897|CAP33FN from Streptococcus pneumoniae (369 aa) FASTA scores: opt: 954, E(): 8.1e-53, (44.8% identity in 375 aa overlap); etc. Cofactor: FAD (by similarity). N-terminal SHOWS similarity to FAD or NAD containing proteins. |
| Functional category | Cell wall and cell processes |
| Proteomics | Identified in the cell membrane fraction of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). Identified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in the membrane protein fraction and whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate (See de Souza et al., 2011). |
| Mutant | Essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 4272276 | 4273475 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3809c|glf
MQPMTARFDLFVVGSGFFGLTIAERVATQLDKRVLVLERRPHIGGNAYSEAEPQTGIEVHKYGAHLFHTSNKRVWDYVRQFTDFTDYRHRVFAMHNGQAYQFPMGLGLVSQFFGKYFTPEQARQLIAEQAAEIDTADAQNLEEKAISLIGRPLYEAFVKGYTAKQWQTDPKELPAANITRLPVRYTFDNRYFSDTYEGLPTDGYTAWLQNMAADHRIEVRLNTDWFDVRGQLRPGSPAAPVVYTGPLDRYFDYAEGRLGWRTLDFEVEVLPIGDFQGTAVMNYNDLDVPYTRIHEFRHFHPERDYPTDKTVIMREYSRFAEDDDEPYYPINTEADRALLATYRARAKSETASSKVLFGGRLGTYQYLDMHMAIASALNMYDNVLAPHLRDGVPLLQDGA
Bibliography
- Weston A et al. [1997]. Biosynthetic origin of mycobacterial cell wall galactofuranosyl residues. Homolog Product Biochemistry Function
- Chen P et al. [1998]. Novel selection for isoniazid (INH) resistance genes supports a role for NAD+-binding proteins in mycobacterial INH resistance. Function
- Mikusová K et al. [2000]. Biosynthesis of the galactan component of the mycobacterial cell wall. Product Biochemistry
- Pan F et al. [2001]. Cell wall core galactofuran synthesis is essential for growth of mycobacteria. Homolog Mutant
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Mawuenyega KG et al. [2005]. Mycobacterium tuberculosis functional network analysis by global subcellular protein profiling. Proteomics
- Beis K et al. [2005]. Crystal structures of Mycobacteria tuberculosis and Klebsiella pneumoniae UDP-galactopyranose mutase in the oxidised state and Klebsiella pneumoniae UDP-galactopyranose mutase in the (active) reduced state. Structure
- Målen H et al. [2010]. Definition of novel cell envelope associated proteins in Triton X-114 extracts of Mycobacterium tuberculosis H37Rv. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
