Gene Rv3824c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Involved in sulfolipid-1 (SL-1) biosynthesis |
| Product | Conserved polyketide synthase associated protein PapA1 |
| Comments | Rv3824c, (MTCY409.06), len: 511 aa. papA1, conserved polyketide synthase (PKS) associated protein, highly similar to Q49618|PAPA3|ML1230|B1170_C1_180 PKS-associated protein A3 from Mycobacterium leprae (471 aa), FASTA scores: opt: 1879, E(): 7.1e-111, (55.5% identity in 465 aa overlap). Also similar to Q9F2R3|SCD65.19c hypothetical 52.8 KDA protein from Streptomyces coelicolor (473 aa), FASTA scores: opt: 476, E(): 1.7e-22, (26.7% identity in 464 aa overlap); and similar in part to Q09164|SIMA|CYSYN cyclosporin synthetase from Tolypocladium inflatum (15281 aa) FASTA scores: opt: 238, E(): 2.8e-06, (22.35% identity in 371 aa overlap). Also highly similar to other PKS-associated proteins from Mycobacterium tuberculosis; O50438|PAPA3|Rv1182|MTV005.18 (472 aa), FASTA scores: opt: 1862, E(): 8.4e-110, (55.95% identity in 470 aa overlap); and upstream ORF O07803|PAPA2|Rv3820c|MTCY409.10 (468 aa) FASTA scores: opt: 1664, E(): 2.5e-97, (53.9% identity in 462 aa overlap). Contains PS00453 FKBP-type peptidyl-prolyl cis-trans isomerase signature 1. |
| Functional category | Lipid metabolism |
| Proteomics | Identified by mass spectrometry in M. tuberculosis H37Rv-infected guinea pig lungs at 90 days but not 30 days (See Kruh et al., 2010). |
| Transcriptomics | mRNA identified by microarray analysis and up-regulated after 4h of starvation (see citation below). DNA microarrays show higher level of expression in M. tuberculosis H37Rv than in phoP|Rv0757 mutant (See Walters et al., 2006). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv and CDC1551 strains (see Sassetti et al., 2003 and Lamichhane et al., 2003). Non-essential gene for in vitro growth of H37Rv, but essential for in vitro growth on cholesterol; by sequencing of Himar1-based transposon mutagenesis (See Griffin et al., 2011). Virulence of M. tuberculosis Erdman mutant in BALB/c mice is unaffected (See Kumar et al., 2007). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 4291639 | 4293174 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3824c|papA1
VRIGPVELSAVKDWDPAPGVLVSWHPTPASCAKALAAPVSAVPPSYVQARQIRSFSEQAARGLDHSRLLIASVEVFGHCDLRAMTYVINAHLRRHDTYRSWFELRDTDHIVRHSIADPADIEFVPTTHGEMTSADLRQHIVATPDSLHWDCFSFGVIQRADSFTFYASIDHLHADGQFVGVGLMEFQSMYTALIMGEPPIGLSEAGSYVDFCVRQHEYTSALTVDSPEVRAWIDFAEINNGTFPEFPLPLGDPSVRCGGDLLSMMLMDEQQTQRFESACMAANARFIGGMLACIAIAIHELTGADTYFGITPKDIRTPADLMTQGWFTGQIPVTVPVAGLSFNEIARIAQTSFDTGADLAKVPFERVVELSPSLRRPQPLFSLVNFFDAQVGPLSAVTKLFEGLNVGTYSDGRVTYPLSTMVGRFDETAASVLFPDNPVARESVTAYLRAIRSVCMRIANGGTAERVGNVVALSPGRRNNIERMTWRSCRAGDFIDICNLKVANVTVDREA
Bibliography
- Betts JC et al. [2002]. Evaluation of a nutrient starvation model of Mycobacterium tuberculosis persistence by gene and protein expression profiling. Transcriptome
- Dahl JL et al. [2003]. The role of RelMtb-mediated adaptation to stationary phase in long-term persistence of Mycobacterium tuberculosis in mice. Regulon
- Lamichhane G et al. [2003]. A postgenomic method for predicting essential genes at subsaturation levels of mutagenesis: application to Mycobacterium tuberculosis. Mutant
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Walters SB et al. [2006]. The Mycobacterium tuberculosis PhoPR two-component system regulates genes essential for virulence and complex lipid biosynthesis. Transcriptome
- Kumar P et al. [2007]. PapA1 and PapA2 are acyltransferases essential for the biosynthesis of the Mycobacterium tuberculosis virulence factor sulfolipid-1. Function Mutant
- Kruh NA et al. [2010]. Portrait of a pathogen: the Mycobacterium tuberculosis proteome in vivo. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
