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virulence, detoxification, adaptation
information pathways
cell wall and cell processes
stable RNAs
insertion seqs and phages
intermediary metabolism and respiration
regulatory proteins
conserved hypotheticals
lipid metabolism
General annotation
FunctionInvolved in sulfolipid-1 (SL-1) biosynthesis
ProductConserved polyketide synthase associated protein PapA1
CommentsRv3824c, (MTCY409.06), len: 511 aa. papA1, conserved polyketide synthase (PKS) associated protein, highly similar to Q49618|PAPA3|ML1230|B1170_C1_180 PKS-associated protein A3 from Mycobacterium leprae (471 aa), FASTA scores: opt: 1879, E(): 7.1e-111, (55.5% identity in 465 aa overlap). Also similar to Q9F2R3|SCD65.19c hypothetical 52.8 KDA protein from Streptomyces coelicolor (473 aa), FASTA scores: opt: 476, E(): 1.7e-22, (26.7% identity in 464 aa overlap); and similar in part to Q09164|SIMA|CYSYN cyclosporin synthetase from Tolypocladium inflatum (15281 aa) FASTA scores: opt: 238, E(): 2.8e-06, (22.35% identity in 371 aa overlap). Also highly similar to other PKS-associated proteins from Mycobacterium tuberculosis; O50438|PAPA3|Rv1182|MTV005.18 (472 aa), FASTA scores: opt: 1862, E(): 8.4e-110, (55.95% identity in 470 aa overlap); and upstream ORF O07803|PAPA2|Rv3820c|MTCY409.10 (468 aa) FASTA scores: opt: 1664, E(): 2.5e-97, (53.9% identity in 462 aa overlap). Contains PS00453 FKBP-type peptidyl-prolyl cis-trans isomerase signature 1.
Functional categoryLipid metabolism
ProteomicsIdentified by mass spectrometry in M. tuberculosis H37Rv-infected guinea pig lungs at 90 days but not 30 days (See Kruh et al., 2010).
TranscriptomicsmRNA identified by microarray analysis and up-regulated after 4h of starvation (see citation below). DNA microarrays show higher level of expression in M. tuberculosis H37Rv than in phoP|Rv0757 mutant (See Walters et al., 2006).
MutantNon-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv and CDC1551 strains (see Sassetti et al., 2003 and Lamichhane et al., 2003). Non-essential gene for in vitro growth of H37Rv, but essential for in vitro growth on cholesterol; by sequencing of Himar1-based transposon mutagenesis (See Griffin et al., 2011). Virulence of M. tuberculosis Erdman mutant in BALB/c mice is unaffected (See Kumar et al., 2007).
Check for mutants available at TARGET website
Genomic sequence
Feature type Upstream flanking region (bp) Downstream flanking region (bp) Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3824c|papA1