Gene Rv3886c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Thought to have proteolytic activity. |
| Product | Probable alanine and proline rich membrane-anchored mycosin MycP2 (serine protease) (subtilisin-like protease) (subtilase-like) (mycosin-2) |
| Comments | Rv3886c, (MTCY15F10.26), len: 550 aa. Probable mycP2, ala-, pro-rich membrane-anchored serine protease (mycosin) (see citation below), highly similar to Q9CBV3|ML1538 possible protease from Mycobacterium leprae (567 aa), FASTA scores: opt: 1034, E(): 3.9e-32, (43.5% identity in 575 aa overlap); and highly similar, but with gaps, to several putative proteases from Mycobacterium leprae; O33076|ML0041|MLCB628.04 (446 aa), FASTA scores: opt: 860, E(): 1.1e-25, (38.65% identity in 538 aa overlap); Q9CD36|ML2528 (475 aa) (475 aa), FASTA scores: opt: 413, E(): 7.1e-09, (37.7% identity in 562 aa overlap). Also similarity with Q99405|PRTM_BACSP M-protease from Bacillus sp. strain KSM-K16 (269 aa), FASTA scores: E(): 7.6e-06, (27.1% identity in 277 aa overlap). And highly similar, but also with gaps, to other mycosins from Mycobacterium tuberculosis e.g. O53945|Rv1796|MTV049.18 (585 aa), FASTA scores: opt: 1173, E(): 2.4e-37, (47.9% identity in 578 aa overlap); the upstream ORF O05461|Rv3883c|MTCY15F10.29 (446 aa) FASTA scores: opt: 910, E(): 1.5e-27, (40.15% identity in 533 aa overlap); O06316|Rv3449|MTCY13E12.02 (455 aa) FASTA scores: opt: 477, E(): 2.7e-11, (38.75% identity in 550 aa overlap); etc. Contains Pro rich protein with two serine protease, subtilase family active site motifs: aspartic acid active site motif (PS00136); and histidine active site motif (PS00137). Belongs to peptidase family S8 (also known as the subtilase family), pyrolysin subfamily. Thought to be cleaved into smaller molecular weight proteins, 36 and 29 KDA (see citation below). |
| Functional category | Intermediary metabolism and respiration |
| Proteomics | Identified in the membrane fraction of M. tuberculosis H37Rv using nanoLC-MS/MS; predicted integral membrane protein (See Xiong et al., 2005). Identified by mass spectrometry in whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate or membrane protein fraction (See de Souza et al., 2011). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv and CDC1551 strains (see Sassetti et al., 2003 and Lamichhane et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 4368518 | 4370170 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3886c|mycP2
MASPLNRPGLRAAAASAALTLVALSANVPAAQAIPPPSVDPAMVPADARPGPDQPMRRSNSCSTPITVRNPDVAQLAPGFNLVNISKAWQYSTGNGVPVAVIDTGVSPNPRLPVVPGGDYIMGEDGLSDCDAHGTVVSSIIAAAPLGILPMPRAMPATAAFPPPAGPPPVTAAPAPPVEVPPPMPPPPPVTITQTVAPPPPPPEDAGAMAPSNGPPDPQTEDEPAVPPPPPGAPDGVVGVAPHATIISIRQSSRAFEPVNPSSAGPNSDEKVKAGTLDSVARAVVHAANMGAKVINISVTACLPAAAPGDQRVLGAALWYAATVKDAVIVAAAGNDGEAGCGNNPMYDPLDPSDPRDWHQVTVVSSPSWFSDYVLSVGAVDAYGAALDKSMSGPWVGVAAPGTHIMGLSPQGGGPVNAYPPSRPGEKNMPFWGTSFSAAYVSGVAALVRAKFPELTAYQVINRIVQSAHNPPAGVDNKLGYGLVDPVAALTFNIPSGDRMAPGAQSRVITPAAPPPPPDHRARNIAIGFVGAVATGVLAMAIGARLRRAR
Bibliography
- Brown GD, Dave JA, Gey Van Pittius NC, Stevens L, Ehlers MR and Beyers AD [2000]. The mycosins of Mycobacterium tuberculosis H37Rv: a family of subtilisin-like serine proteases. Product Localization
- Gey Van Pittius NC, Gamieldien J, Hide W, Brown GD, Siezen RJ and Beyers AD [2001]. The ESAT-6 gene cluster of Mycobacterium tuberculosis and other high G+C Gram-positive bacteria. Secondary Phylogeny
- Lamichhane G et al. [2003]. A postgenomic method for predicting essential genes at subsaturation levels of mutagenesis: application to Mycobacterium tuberculosis. Mutant
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Xiong Y, Chalmers MJ, Gao FP, Cross TA and Marshall AG [2005]. Identification of Mycobacterium tuberculosis H37Rv integral membrane proteins by one-dimensional gel electrophoresis and liquid chromatography electrospray ionization tandem mass spectrometry. Proteomics
- [2009]. Systematic genetic nomenclature for type VII secretion systems. Nomenclature
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
