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virulence, detoxification, adaptation
information pathways
cell wall and cell processes
stable RNAs
insertion seqs and phages
PE/PPE
intermediary metabolism and respiration
unknown
regulatory proteins
conserved hypotheticals
lipid metabolism
pseudogenes
General annotation
TypeCDS
FunctionThought to have proteolytic activity.
ProductProbable alanine and proline rich membrane-anchored mycosin MycP2 (serine protease) (subtilisin-like protease) (subtilase-like) (mycosin-2)
CommentsRv3886c, (MTCY15F10.26), len: 550 aa. Probable mycP2, ala-, pro-rich membrane-anchored serine protease (mycosin) (see citation below), highly similar to Q9CBV3|ML1538 possible protease from Mycobacterium leprae (567 aa), FASTA scores: opt: 1034, E(): 3.9e-32, (43.5% identity in 575 aa overlap); and highly similar, but with gaps, to several putative proteases from Mycobacterium leprae; O33076|ML0041|MLCB628.04 (446 aa), FASTA scores: opt: 860, E(): 1.1e-25, (38.65% identity in 538 aa overlap); Q9CD36|ML2528 (475 aa) (475 aa), FASTA scores: opt: 413, E(): 7.1e-09, (37.7% identity in 562 aa overlap). Also similarity with Q99405|PRTM_BACSP M-protease from Bacillus sp. strain KSM-K16 (269 aa), FASTA scores: E(): 7.6e-06, (27.1% identity in 277 aa overlap). And highly similar, but also with gaps, to other mycosins from Mycobacterium tuberculosis e.g. O53945|Rv1796|MTV049.18 (585 aa), FASTA scores: opt: 1173, E(): 2.4e-37, (47.9% identity in 578 aa overlap); the upstream ORF O05461|Rv3883c|MTCY15F10.29 (446 aa) FASTA scores: opt: 910, E(): 1.5e-27, (40.15% identity in 533 aa overlap); O06316|Rv3449|MTCY13E12.02 (455 aa) FASTA scores: opt: 477, E(): 2.7e-11, (38.75% identity in 550 aa overlap); etc. Contains Pro rich protein with two serine protease, subtilase family active site motifs: aspartic acid active site motif (PS00136); and histidine active site motif (PS00137). Belongs to peptidase family S8 (also known as the subtilase family), pyrolysin subfamily. Thought to be cleaved into smaller molecular weight proteins, 36 and 29 KDA (see citation below).
Functional categoryIntermediary metabolism and respiration
ProteomicsIdentified in the membrane fraction of M. tuberculosis H37Rv using nanoLC-MS/MS; predicted integral membrane protein (See Xiong et al., 2005). Identified by mass spectrometry in whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate or membrane protein fraction (See de Souza et al., 2011).
MutantNon-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv and CDC1551 strains (see Sassetti et al., 2003 and Lamichhane et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011).
Check for mutants available at TARGET website
Coordinates
TypeStartEndOrientation
CDS43685184370170-
Genomic sequence
Feature type Upstream flanking region (bp) Downstream flanking region (bp) Update
       
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3886c|mycP2
MASPLNRPGLRAAAASAALTLVALSANVPAAQAIPPPSVDPAMVPADARPGPDQPMRRSNSCSTPITVRNPDVAQLAPGFNLVNISKAWQYSTGNGVPVAVIDTGVSPNPRLPVVPGGDYIMGEDGLSDCDAHGTVVSSIIAAAPLGILPMPRAMPATAAFPPPAGPPPVTAAPAPPVEVPPPMPPPPPVTITQTVAPPPPPPEDAGAMAPSNGPPDPQTEDEPAVPPPPPGAPDGVVGVAPHATIISIRQSSRAFEPVNPSSAGPNSDEKVKAGTLDSVARAVVHAANMGAKVINISVTACLPAAAPGDQRVLGAALWYAATVKDAVIVAAAGNDGEAGCGNNPMYDPLDPSDPRDWHQVTVVSSPSWFSDYVLSVGAVDAYGAALDKSMSGPWVGVAAPGTHIMGLSPQGGGPVNAYPPSRPGEKNMPFWGTSFSAAYVSGVAALVRAKFPELTAYQVINRIVQSAHNPPAGVDNKLGYGLVDPVAALTFNIPSGDRMAPGAQSRVITPAAPPPPPDHRARNIAIGFVGAVATGVLAMAIGARLRRAR
      
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