Gene Rv3894c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Unknown |
| Product | ESX conserved component EccC2. ESX-2 type VII secretion system protein. Possible membrane protein. |
| Comments | Rv3894c, (MTCY15F10.18), len: 1396 aa. EccC2, esx conserved component, ESX-2 type VII secretion system protein, possible membrane protein (possible transmembrane segments from aa ~37-85), similar to Q9CD30|ML2535 hypothetical protein from Mycobacterium leprae (1329 aa), FASTA scores: opt: 652, E(): 2.2e-30, (27.85% identity in 1425 aa overlap); Q9CDD7|ML0052 hypothetical protein from Mycobacterium leprae (597 aa), FASTA scores: opt: 537, E(): 6.6e-24, (27.5% identity in 585 aa overlap) (similarity only with C-terminal end); Q9Z5I2|ML1543|MLCB596.28 possible SPOIIIE-family membrane protein from Mycobacterium leprae (1345 aa), FASTA scores: opt: 523, E(): 8.6e-23, (31.65% identity in 1412 aa overlap). Also similar to various proteins e.g. O86653|SC3C3.20c ATP/GTP binding protein from Streptomyces coelicolor (1321 aa), FASTA scores: opt: 973, E(): 2.8e-49, (28.1% identity in 1409 aa); Q9L0T6|SCD35.15c putative cell division-related protein from Streptomyces coelicolor(1525 aa), FASTA scores: opt: 524, E(): 8.3e-23, (24.95% identity in 1450 aa overlap); Q9KE81|BH0975 hypothetical protein from Bacillus halodurans (1489 aa), FASTA scores: opt: 444, E(): 4.1e-18, (22.5% identity in 1346 aa overlap); etc. Also similar to AAK46103|MT1833 FTSK/SPOIIIE family protein from Mycobacterium tuberculosis strain CDC1551 (1391 aa), FASTA scores: opt: 769, E(): 2.9e-37, (30.6% identity in 1434 aa overlap); and other hypothetical proteins from Mycobacterium tuberculosis e.g. O53689|Rv0284|MTV035.12 (1330 aa), FASTA scores: opt: 634, E(): 2.5e-29, (28.2% identity in 1443 aa overlap); O06264|Rv3447c|MTCY77.19c (1236 aa), FASTA scores: opt: 632, E(): 3.1e-29, (28.75% identity in 1391 aa overlap); O69736|R3871|MTV027.06 (591 aa), FASTA scores: opt: 588, E(): 6.6e-27, (27.75% identity in 605 aa overlap) (similarity only with C-terminal end); etc. Contains two possible (PS00017) ATP/GTP-binding sites (P-loop) in central portion. |
| Functional category | Cell wall and cell processes |
| Proteomics | Identified in the cell membrane fraction of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). Identified by mass spectrometry in M. tuberculosis H37Rv-infected guinea pig lungs at 30 days but not 90 days (See Kruh et al., 2010). Identified by mass spectrometry in whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate or membrane protein fraction (See de Souza et al., 2011). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv and CDC1551 strains (see Sassetti et al., 2003 and Lamichhane et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 4376262 | 4380452 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3894c|eccC2
MSKKAFPINRVNIDPPKPVRVAPNPPIALPEREPRNIWVMIGVPALIVALIGTIVMLYVSGVRSLATGFFPLMGIGAFSMLAFSGRFGRARKITWGELEKGRRRYLRDLDTNRDEIQTAVCAQREWQNAVHSDPPGLGAIIGGPRMWERGRGDVDFLEVRVGTGVQHAPDSVLSVTWPDISSDEELEPVTGQALRDFILEQRKIRDIAKVVNLRSAPGFSFVSEDLDRVRSLMRSVLCSLAVFHNPRDVKLMVVTRNREVWAWMVWLPHNLHDELFDACGWRRLIFATPEELEAALGAELHMKGKRGAWTPPTVASPTAMGSALETGQVGVDLGPHLVIVDDNTGSPDAWESVVGQVGKAGLTVLRIASRVGTGVGFAEDQVFEMAQRHGAATAVKAGRDGADADDDQRPAPLLRARGTFFAHADQLSIHRAYRYARAMARWSPTSRSEVTDSTSGAAELLRSLGISDPRELDVDRLWAERRGRGDDRWCEIPVGAKPNGELQNIILRAKDFGGFGFHSVVIGTSGSGKSELFLSLVYGIALTHSPETFNVIFVDMKFESAAQDILGIPHVVAALSNLGKDERHLAERMRRVIDGEIKQRYELFKSVGARDANDYEEIRLAGRDLPPVPVLLVIVDEYLELFANHKKWIDLIIHIGQEGRGANVFFMLGGQRLDLSSLQKVKSNIAFRIALRAESGDDSREVIGSDAAYHLPSKENGFALLKVGPRDLEPFRCFYLSAPFVVPKKKEVARTIDMTLTQPRLYDWQYQPLDAADAEALATAAAADAEPDEFLYYDDGFKKKKIVDVLRESLYNVPHRSPRRPWLAPLEDPEPVDRLVAAYRGKPWHVDYGQNPGLMFPVGVMDIPEESQQVVHAVDALRSNIIVVGAKQRGKTTTLMALMCSAATMYTPERVTFFCIGGATMAQIGSLPHVTDIVSPKDAEGIERILSTMDALIDAREEAFRRAKIDMDGFRERRFGIGGDGVGGTDPTDAFGDVFVVLDDYDDLYAKDTLLGDRIISLSSRGPEYGVHLMCSAGGWIHGQRQSLLQNVTARIQLRLADPGESQMGHLSIESREAARRTLNRPGFGLTESLHELRIGVPALADPGTGELVGITDVGARIADVAGVTKHASLQRLPQRVELSAIVEHEAVHQGGDDLSIAFAIGERHELGPVPIKLRESPGLMILGRQGCGKTTALVAIGEAVMNRFSPQQAQLTLIDPKTAPHGLRDLHAPGYVRAYAYDQDEIDEVITELAQQILLPRLPPKGLSQEELRALKPWEGPRHFVLIDDVQDLRPAQSYPQKPPVGAALWKLMERARQVGLHVFSTRNSANWATMPMDPWVKSQTSAKVAQLYMDNDPQNRINRSVRAQTLPPGRGLLVGADGDVEGILVGYPSVPGEQ
Bibliography
- Gey Van Pittius NC, Gamieldien J, Hide W, Brown GD, Siezen RJ and Beyers AD [2001]. The ESAT-6 gene cluster of Mycobacterium tuberculosis and other high G+C Gram-positive bacteria. Secondary
- Lamichhane G et al. [2003]. A postgenomic method for predicting essential genes at subsaturation levels of mutagenesis: application to Mycobacterium tuberculosis. Mutant
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Mawuenyega KG et al. [2005]. Mycobacterium tuberculosis functional network analysis by global subcellular protein profiling. Proteomics
- [2009]. Systematic genetic nomenclature for type VII secretion systems. Nomenclature
- Kruh NA et al. [2010]. Portrait of a pathogen: the Mycobacterium tuberculosis proteome in vivo. Proteomics
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
