Gene Rv3918c (parB)
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Involved in chromosome partition. Localize to both POLES of the predivisional cell following completion of DNA replication. |
| Product | Probable chromosome partitioning protein ParA |
| Comments | Rv3918c, (MTV028.09c), len: 347 aa. Probable parA, chromosome partitioning protein, highly similar to Q9CCX7|para|ML2707 putative cell division protein from Mycobacterium leprae (351 aa), FASTA scores: opt: 1679, E(): 2.9e-93, (78.1% identity in 347 aa overlap). Also highly similar to others e.g. Q9RFM1|para para protein from Streptomyces coelicolor (357 aa), FASTA scores: opt: 1197, E(): 2e-64, (60.45% identity in 306 aa overlap); Q98DZ3|MLL4479|para chromosome partitioning protein from Rhizobium loti (Mesorhizobium loti) (266 aa), FASTA scores: opt: 835, E(): 7.2e-43, (50.95% identity in 257 aa overlap); O05189|PARA_CAUCR chromosome partitioning protein from Caulobacter crescentus (267 aa), FASTA scores: opt: 813, E(): 1.5e-41, (51.35% identity in 261 aa overlap) (has its N-terminus shorter); etc. Equivalent to AAK48403 from Mycobacterium tuberculosis strain CDC1551 (381 aa) but shorter 34 aa. Also similar to other Mycobacterium tuberculosis proteins: MTCI125.30, FASTA scores: E(): 4.3e-32, (35.2% identity in 327 aa overlap); and MTCY07D11.13, FASTA scores: E(): 3e-30, (39.9% identity in 263 aa overlap). Belongs to the para family. Possible alternative start site at aa 107. Note that previously known as parB. |
| Functional category | Cell wall and cell processes |
| Proteomics | Identified by mass spectrometry in whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate or membrane protein fraction (See de Souza et al., 2011). Translational start site supported by proteomics data (See Kelkar et al., 2011). |
| Transcriptomics | mRNA identified by microarray analysis and down-regulated after 4h and 96h of starvation (see citation below). |
| Mutant | Essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 4406488 | 4407531 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3918c|parA
VSAPWGPVAAGPSALVRSGQASTIEPFQREMTPPTPTPEAAHNPTMNVSRETSTEFDTPIGAAAERAMRVLHTTHEPLQRPGRRRVLTIANQKGGVGKTTTAVNIAAALAVQGLKTLVIDLDPQGNASTALGITDRQSGTPSSYEMLIGEVSLHTALRRSPHSERLFCIPATIDLAGAEIELVSMVARENRLRTALAALDNFDFDYVFVDCPPSLGLLTINALVAAPEVMIPIQCEYYALEGVSQLMRNIEMVKAHLNPQLEVTTVILTMYDGRTKLADQVADEVRQYFGSKVLRTVIPRSVKVSEAPGYSMTIIDYDPGSRGAMSYLDASRELAERDRPPSAKGRP
Bibliography
- Betts JC et al. [2002]. Evaluation of a nutrient starvation model of Mycobacterium tuberculosis persistence by gene and protein expression profiling. Transcriptome
- Dahl JL et al. [2003]. The role of RelMtb-mediated adaptation to stationary phase in long-term persistence of Mycobacterium tuberculosis in mice. Regulon
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- Kelkar DS et al. [2011]. Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry. Proteomics Sequence
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
