Gene ML0321 in Mycobacterium leprae TN
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virulence, detoxification, adaptation
information pathways
cell wall and cell processes
stable RNAs
insertion seqs and phages
PE/PPE
intermediary metabolism and respiration
unknown
regulatory proteins
conserved hypotheticals
lipid metabolism
pseudogenes
Gene summary information
Gene nameispD
Gene length726 bp
IdentifierML0321
Location 410871 bp
Protein summary information
Molecular mass24980.0 Da
Isoelectric point8.93
Protein length242 amino acids
Structural information
PFAMPF01128
Orthologs
M. tuberculosis H37RvRv3582c
M. lepromatosis Mx1-22AMLPM_0321
Cross-references
UniProtQ9CCW6
Gene OntologyTerpenoid biosynthetic process, 2-c-methyl-d-erythritol 4-phosphate cytidylyltransferase activity
Enzyme Classification2.7.7.60
Precomputed results
BLASTReport
General annotation
TypeCDS
FunctionInvolved in the terpenoid biosynthesis pathway (at the third step). Catalyzes the formation of 4-diphosphocytidyl-2C-methyl-D-erythritol from 2C-methyl-D-erythritol 4-phosphate. [Catalytic activity: CTP and CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol].
ProductProbable 4-diphosphocytidyl-2C-methyl-D-erythritol synthase IspD (2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase) (MEP cytidylyltransferase) (MCT)
CommentsML0321, len: 241 aa. Probable ispD, 4-diphosphocytidyl-2C-methyl-D-erythritol synthase (EC 2.7.7.-), highly similar to Rv3582c|YZ82_MYCTU|P96864 probable ispD, 4-diphosphocytidyl-2C-methyl-D-erythritol synthase from M. tuberculosis (231 aa), Fasta scores: E(): 0, (66.0% identity in 241 aa overlap). Similar to other bacterial 4-diphosphocytidyl-2C-methyl-D-erythritol synthases e.g. CAB77327|AL160331 from Streptomyces coelicolor (270 aa), Fasta scores: E(): 4.3e-25, (45.0% identity in 240 aa overlap) and AAF43207|AF230736 ispD from Escherichia coli (236 aa), Fasta scores: E(): 0.0016, (36.1% identity in 244 aa overlap). Contains the PS01295 4-diphosphocytidyl-2C-methyl-D-erythritol synthase signature and Pfam match to entry PF01128 UPF0007, Uncharacterized protein family UPF0007. Belongs to the IspD family.
Functional categoryIntermediary metabolism and respiration
Mutant
Check for mutants available at TARGET website
Coordinates
TypeStartEndOrientation
CDS410871411596+
Genomic sequence
Feature type Upstream flanking region (bp) Downstream flanking region (bp) Update
Protein sequence
>Mycobacterium leprae TN|ML0321|ispD
MAVATGTVVAVVPAAGAGKRLAAGIPKAFCELDGRTLVERAVVGLLESGVVDHVVVAVPADRIAQTQWVLSQRLANSAGQHATVVAGGADRTKSVCQALATLPAPSRVGAPEFILVHDAARALTPARLIVRVVDALRAGHTAVVPALPLSDTIKAVDANGMVLGTPARVGLRAVQTPQGFATELLWCAYQRGPHLDAVDFTDDASLVEHLGGQVQVVAGDPLAFKITTQLDLLLAKKILRR
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