Gene ML0481
in Mycobacterium leprae TN
General annotation
| Type | CDS |
| Function | NUCLEASE THAT RESOLVES HOLLIDAY JUNCTION INTERMEDIATES IN GENETIC RECOMBINATION. CLEAVES THE CRUCIFORM STRUCTURE IN SUPERCOILED DNA BY NICKING TO STRANDS WITH THE SAME POLARITY AT SITES SYMMETRICALLY OPPOSED AT THE JUNCTION IN THE HOMOLOGOUS ARMS AND LEAVES A 5'TERMINAL PHOSPHATE AND A 3'TERMINAL HYDROXYL GROUP [CATALYTIC ACTIVITY: ENDONUCLEOLYTIC CLEAVAGE AT A JUNCTION SUCH AS A RECIPROCAL SINGLE-STRANDED CROSSOVER BETWEEN TWO HOMOLOGOUS DNA DUPLEXES (HOLLIDAY JUNCTION)]. |
| Product | Probable crossover junction endodeoxyribonuclease RuvC (HOLLIDAY JUNCTION NUCLEASE) (HOLLIDAY JUNCTION RESOLVASE). |
| Comments | ML0481, len: 188 aa. Probable ruvC, Holliday junction resolvase (EC 3.1.22.4) (see citations below), highly similar to RUVC_MYCTU|Q50627|Rv2594c ruvC, crossover junction endodeoxyribonuclease from M. tuberculosis (188 aa), Fasta scores: E(): 0, (81.5% identity in 184 aa overlap). Also highly similar to others e.g. Q9AE11|RUVC from Corynebacterium glutamicum (Brevibacterium flavum) (221 aa), fasta scores: E(): 9.7e-41, (57.672% identity in 189 aa overlap); and Q9L289|RUVC_STRCO|SCL2.10c from Streptomyces coelicolor (188 aa), fasta scores: E(): 4.2e-39, (64.286% identity in 168 aa overlap). Previously sequenced as RUVC_MYCLE|P40834 (188 aa), Fasta scores: E(): 0, (100.0% identity in 188 aa overlap). Contains Pfam match to entry PF02075 RuvC, Crossover junction endodeoxyribonuclease RuvC. Contains PS01321 Crossover junction endodeoxyribonuclease RuvC signature. Contains PS00572 Glycosyl hydrolases family 1 active site. |
| Functional category | Information pathways |
| Mutant | Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 581225 | 581791 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium leprae TN|ML0481|ruvC
MRVMGVDPGLTRCGLSVVENGRGSQVVALDVDVVRTPSDAPVSKRLLAVSDVVEHWLDAHHPDVMAIERVFSQQNVSTVMGTAQAGGVIALAAARRGIDVHFHTPSEVKAAVTGNGAANKAQVTAMVTRILALQAKPTPADAADALALAICHCWRAPMIARMAEAEALGARHRQAYRAKVAGEVKATR
Bibliography
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