Gene ML1245
in Mycobacterium leprae TN
General annotation
| Type | CDS |
| Function | C-TERMINUS: INVOLVED IN PHOSPHOLIPID BIOSYNTHESIS (AT THE SECOND STEP); CONVERTS LYSOPHOSPHATIDIC ACID (LPA) INTO PHOSPHATIDIC ACID BY INCORPORATING ACYL MOIETY AT THE 2 POSITION [CATALYTIC ACTIVITY 2: ACYL-CoA + 1-ACYL-SN-GLYCEROL 3-PHOSPHATE = CoA + 1,2-DIACYL-SN-GLYCEROL 3-PHOSPHATE]. N-TERMINUS: COULD BE GENERATE SERINE AND PHOSPHATE FROM PHOSPHOSERINE; MAY CATALYZE THE LAST STEP IN THE BIOSYNTHESIS OF SERINE FROM CARBOHYDRATES (THE REACTION MECHANISM COULD BE PROCEED VIA THE FORMATION OF A PHOSPHORYL-ENZYME INTERMEDIATES) [CATALYTIC ACTIVITY 1: PHOSPHOSERINE + H(2)O = SERINE + PHOSPHATE]. |
| Product | POSSIBLE TRANSMEMBRANE PHOSPHOLIPID BIOSYNTHESIS BIFUNCTIONAL ENZYME PLSC: PUTATIVE L-3-PHOSPHOSERINE PHOSPHATASE (O-PHOSPHOSERINE PHOSPHOHYDROLASE) (PSP) (PSPASE) + 1-ACYL-SN-GLYCEROL-3-PHOSPHATE ACYLTRANSFERASE (1-AGP ACYLTRANSFERASE) (1-AGPAT) (LYSOPHOSPHATIDIC ACID ACYLTRANSFERASE) (LPAAT) |
| Comments | ML1245, len: 579 aa. Possible plsC, a transmembrane phospholipid biosynthesis bifunctional enzyme, including L-3-phosphoserine phosphatase (EC 3.1.3.3) and 1-acyl-Sn-glycerol-3-phosphate acyltransferase (EC 2.3.1.51). The N-terminus is similar to several phosphoserine phosphatases e.g. Haemophilus influenzae SW:SERB_HAEIN (P44997) (795 aa), BlastP Expect 9.8 and the C-terminus is similar to many Prokaryotic and Eukaryotic acyltransferases e.g. Salmonella typhimurium 1-acyl-SN-glycerol-3-phosphate acyltransferase SW:PLSC_SALTY (P26974) (245 aa), Fasta scores: E(): 1.4e-11, 35.3% identity in 167 aa overlap. Highly similar over entire protein to Mycobacterium tuberculosis putative transferase Rv2483c TR:O53208 (EMBL:AL021246) (580 aa), Fasta scores: E(): 0, 77.1% identity in 573 aa overlap. Contains Pfam match to entry PF01553 Acyltransferase, Acyltransferase. |
| Functional category | Lipid metabolism |
| Mutant | Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 1484292 | 1486031 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium leprae TN|ML1245|plsC
MSAFDENGTPKSGPEVRLPGSVAEILASPAGPQVGAFFDLDGTLVAGFTAVILTHERLRRCDMGVGELLSMIQAGLNHTLGRIEFEDLIGKVSSALRGRLLTDLEEIGERLFAQRIESRIYPEMRKLVQAHVARGHTVVLSSSALTIQVGPVARFLGIAHMLTNKFEINEDGMLTGGVVKPILWGPGKAAAVQRFAAEHDIDLKDSYFYADGDEDVALMYLVGNPRPTNPEGKMAAVAKRRGWPILKFNSRGGVGIGAQLRTLVGLSSVLPLVAGAVGIGVLTGSRRRGANFFTSVFPPLVLAAAGVHLNVIGNENLTMQRPAVFIYNHRNQIDPLIAGALVRGNWIAVAKKELAKNPIIRALGKLTDGVFIDRDDPVGAVETLHAVEDRARKGLSILMAPEGTRLDTTEVGPFKKGPFRIAMAVGIPIVPIVFRNAEIVAARNSNTINPGMVDVAVLPAIPVDDWTLDALSERIAEVRQLYLDILADWPVDELPKAALYTRATKATVKKAEPKKGGRTSAAKTTAKKTATKATAAKTANTAVTKSMLTPPTRISDKENPKVPKVVSQDLVVKQPRERS
Bibliography
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