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virulence, detoxification, adaptation
information pathways
cell wall and cell processes
stable RNAs
insertion seqs and phages
PE/PPE
intermediary metabolism and respiration
unknown
regulatory proteins
conserved hypotheticals
lipid metabolism
pseudogenes
General annotation
TypeCDS
FunctionC-terminus: involved in phospholipid biosynthesis (at the second step); converts lysophosphatidic acid (LPA) into phosphatidic acid by incorporating acyl moiety at the 2 position [catalytic activity 2: acyl-CoA + 1-acyl-SN-glycerol 3-phosphate = CoA + 1,2-diacyl-SN-glycerol 3-phosphate]. N-terminus: could be generate serine and phosphate from phosphoserine; may catalyze the last step in the biosynthesis of serine from carbohydrates (the reaction mechanism could be proceed via the formation of a phosphoryl-enzyme intermediates) [catalytic activity 1: phosphoserine + H(2)O = serine + phosphate].
ProductPossible transmembrane phospholipid biosynthesis bifunctional enzyme PlsC: putative L-3-phosphoserine phosphatase (O-phosphoserine phosphohydrolase) (PSP) (pspase) + 1-acyl-SN-glycerol-3-phosphate acyltransferase (1-AGP acyltransferase) (1-AGPAT) (lysophosphatidic acid acyltransferase) (LPAAT)
CommentsRv2483c, (MTV008.39c), len: 580 aa. Possible plsC, a transmembrane phospholipid biosynthesis bifunctional enzyme, including L-3-phosphoserine phosphatase and 1-acyl-Sn-glycerol-3-phosphate acyltransferase , equivalent to Q9X7A9|PLSC|ML1245 putative acyltransferase from Mycobacterium leprae (579 aa), FASTA scores: opt: 2835, E(): 9.2e-153, (77.15% identity in 573 aa overlap). C-terminal end is similar to many 1-acyl-SN-glycerol-3-phosphate acyltransferases (lysophosphatidic acidacyltransferases) e.g. Q9SDQ2 from Limnanthes floccosa (281 aa), FASTA scores: opt: 378, E(): 3.1e-14, (30.0% identity in 230 aa overlap) and Q42868|PLSC_LIMAL from Limnanthes alba (White meadowfoam) (281 aa), FASTA scores: opt: 374, E(): 5.2e-14, (30.55% identity in 221 aa overlap); and the N-terminal end is similar to many SerB family proteins e.g. AAK44749|MT0526 from Mycobacterium tuberculosis strain CDC1551 (308 aa), FASTA scores: opt: 356, E(): 5.8e-13, (32.5% identity in 298 aa overlap) and Q49823|ML2424 from Mycobacterium leprae (300 aa), FASTA scores: opt: 346, E(): 2.1e-12, (32.0% identity in 278 aa overlap). So belongs to the 1-acyl-SN-glycerol-3-phosphate acyltransferase family and may belong to the SerB family.
Functional categoryLipid metabolism
ProteomicsIdentified in the membrane fraction of M. tuberculosis H37Rv using 1D-SDS-PAGE and uLC-MS/MS (See Gu et al., 2003). Identified in the cell membrane fraction of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005).
MutantNon-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Required for growth in C57BL/6J mouse spleen, by transposon site hybridization (TraSH) in H37Rv (See Sassetti and Rubin, 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011).
Check for mutants available at TARGET website
Coordinates
TypeStartEndOrientation
CDS27892802791022-
Genomic sequence
Feature type Upstream flanking region (bp) Downstream flanking region (bp) Update
       
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv2483c|plsC
MSAADEQGEERATRKSAPDLRLPGSVAEILASPAGPKVGAFFDLDGTLVAGFTAVILTQERLRRRDMGVGELLGMVQAGLNHTLGRIEFEDLIGKAAAALAGRLLTDLEEIGERLFAQRIESRIYPEMRELVRAHVARGHTVVLSSSALTIQVGPVARFLGINNMLTNKFETNEDGILTGGVLKPILWCPGKATAVQRFAAEHDIDLKDSYFYADGDEDVALMYLVGNPRPTNPEGKMAAVAKRRGWPILKFNSRGGVGIRRQLRTLAGLSTIVPVAAGAVGIGVLTGSRRRGVNFFTSTFSQLLLATSGVHLNVIGKENLTAQRPAVFIFNHRNQVDPVIAGALVRDNWVGVGKKELASDPIMGTLGKLLDGVFIDRDDPVAAVETLHTVEERARNGLSIVIAPEGTRLDTTEVGSFKKGPFRIAMAAKIPIVPIVIRNAEIVASRNSTTINPGTVDVAVFPPIPVDDWTLDALPDRIAEVRQLYLDTLADWPVDGLPAVDLYAEQKAARKARAQVAKATAKRVPAKKAPAKSAANKGAAATKAATKKASPKAKPSESKIAGKDGEASASPSSSAKGRS