Gene Mb0051
in Mycobacterium bovis AF2122/97
General annotation
| Type | CDS |
| Function | Unknown |
| Product | PROBABLE BIFUNCTIONAL PENICILLIN-BINDING PROTEIN 1A/1B PONA1 (MUREIN POLYMERASE) (PBP1): PENICILLIN-INSENSITIVE TRANSGLYCOSYLASE (PEPTIDOGLYCAN TGASE) + PENICILLIN-SENSITIVE TRANSPEPTIDASE (DD-TRANSPEPTIDASE) |
| Comments | Mb0051, ponA1, len: 822 aa. Equivalent to penicillin-binding protein, len: 822 aa, from Mycobacterium bovis strain AN5, (99% identity in 821 aa overlap), Equivalent to penicillin-binding protein from Mycobacterium bovis BCG strain Korea 1168P (99% identity in 821 aa overlap), penicillin-binding protein from Mycobacterium tuberculosis strain CDC1551 (99% identity in 817 aa overlap), bifunctional penicillin-binding protein 1A/1B ponA1: penicillin-insensitive transglycosylase + penicillin-sensitive transpeptidase from Mycobacterium tuberculosis strain F11 (99% identity with 819 aa overlap). Probable ponA1, penicillin-binding protein (class A), bienzymatic protein with transglycosylase (EC 2.4.2.-) and transpeptidase (EC 3.4.-.-) activities,highly similar to many e.g. NP_302715.1|NC_002677 penicillin-binding protein from Mycobacterium leprae (708 aa); AAB53123.1|L39923 penicillin binding protein from Mycobacterium leprae (686 aa), FASTA scores: (82.3% identity in 679 aa overlap); Q9F9V7|PONA|AAG13121.1|AF165523_1|AF165523 penicillin-binding protein 1 from Mycobacterium smegmatis (715 aa) (see citation below); CAB88838.1|AL353832 probable penicillin-binding protein from Streptomyces coelicolor (756 aa); etc. Also similar to ponA2|Rv3682|MTV025.030 BIFUNCTIONAL MEMBRANE-ASSOCIATED PENICILLIN-BINDING PROTEIN 1A/1B from Mycobacterium tuberculosis (810 aa). BELONGS TO THE TRANSGLYCOSYLASE FAMILY IN THE N-TERMINAL SECTION, AND TO THE TRANSPEPTIDASE FAMILY IN THE C-TERMINAL SECTION. UPDATE: 5' annotation extension based on de novo proteomics from Mycobacterium tuberculosis strain H37Rv under exponential conditions (See Schubert et al., 2013). Mb0051 transcript and transcriptional start site identified in Mycobacterium bovis strain AF2122/97 grown under exponential conditions. |
| Functional category | |
| Mutant | Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 53221 | 55689 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium bovis AF2122-97|Mb0051|ponA1
MNSDGRHHQSSSGAPRGPANPGQRGQVPPDDRLTAILPPVTDDRSAPHADSIEAVKAALDGAPPMPPPRDPLEEVTAALAAPPGKPPRGDQLGGRRRPPGPPGPPGSSGQPAGRLPQPRVDLPRVGQINWKWIRRSLYLTAAVVILLLMVTFTMAYLIVDVPKPGDIRTNQVSTILASDGSEIAKIVPPEGNRVDVNLSQVPMHVRQAVIAAEDRNFYSNPGFSFTGFARAVKNNLFGGDLQGGSTITQQYVKNALVGSAQHGWSGLMRKAKELVIATKMSGEWSKDDVLQAYLNIIYFGRGAYGISAASKAYFDKPVEQLTVAEGALLAALIRRPSTLDPAVDPEGAHARWNWVLDGMVETKALSPNDRAAQVFPETVPPDLARAENQTKGPNGLIERQVTRELLELFNIDEQTLNTQGLVVTTTIDPQAQRAAEKAVAKYLDGQDPDMRAAVVSIDPHNGAVRAYYGGDNANGFDFAQAGLQTGSSFKVFALVAALEQGIGLGYQVDSSPLTVDGIKITNVEGEGCGTCNIAEALKMSLNTSYYRLMLKLNGGPQAVADAAHQAGIASSFPGVAHTLSEDGKGGPPNNGIVLGQYQTRVIDMASAYATLAASGIYHPPHFVQKVVSANGQVLFDASTADNTGDQRIPKAVADNVTAAMEPIAGYSRGHNLAGGRDSAAKTGTTQFGDTTANKDAWMVGYTPSLSTAVWVGTVKGDEPLVTASGAAIYGSGLPSDIWKATMDGALKGTSNETFPKPTEVGGYAGVPPPPPPPPSEVPPSETVIQPTVEIAPGITIPIGPPTTITLAPPPPAPPAATPTPPP
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