Gene Mb0059
in Mycobacterium bovis AF2122/97
General annotation
| Type | CDS |
| Function | Unknown |
| Product | PROBABLE REPLICATIVE DNA HELICASE DNAB |
| Comments | Mb0059, dnaB, len: 874 aa. Equivalent to Rv0058,len: 874 aa, from Mycobacterium tuberculosis strain H37Rv,(99.9% identity in 874 aa overlap). Probable dnaB,replicative DNA helicase (EC 3.6.1.-). Contains an intein (position 61630..62838) similar to, and in the same position as, those in Sycnechocystis and Rhodothermus marinus (see citation below). Highly similar to others e.g. DNAB_SYNY3|Q55418 replicative dna helicase (872 aa),FASTA scores: opt: 533, E(): 0, (32.5% identity in 424 aa overlap). Also similar to intein recA|E1173867|AL008967 RECA INTEIN from Mycobacterium tuberculosis (442 aa),FASTA scores: E(): 3.8e-16, (27.0% identity in 426 aa overlap). C-terminal extein (position 62839..63015) similar to many dnaB proteins e.g. NP_302709.1|NC_002677|P46394|DNAB_MYCLE REPLICATIVE DNA HELICASE from Mycobacterium leprae (604 aa); DNAB_ECOLI|P03005 replicative dna helicase from Escherichia coli (471 aa), FASTA scores: opt: 148, E(): 1.5e-07, (37.9% identity in 58 aa overlap); etc. THIS PROTEIN UNDERGOES A PROTEIN SELF SPLICING THAT INVOLVES A POST-TRANSLATIONAL EXCISION OF THE INTERVENING REGION (INTEIN) FOLLOWED BY PEPTIDE LIGATION. BELONGS TO THE HELICASE FAMILY, DNAB SUBFAMILY. IN THE INTEIN SECTION; BELONGS TO THE HOMING ENDONUCLEASE FAMILY. |
| Functional category | Information pathways |
| Mutant | Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 60386 | 63010 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium bovis AF2122-97|Mb0059|dnaB
MAVVDDLAPGMDSSPPSEDYGRQPPQDLAAEQSVLGGMLLSKDAIADVLERLRPGDFYRPAHQNVYDAILDLYGRGEPADAVTVAAELDRRGLLRRIGGAPYLHTLISTVPTAANAGYYASIVAEKALLRRLVEAGTRVVQYGYAGAEGADVAEVVDRAQAEIYDVADRRLSEDFVALEDLLQPTMDEIDAIASSGGLARGVATGFTELDEVTNGLHPGQMVIVAARPGVGKSTLGLDFMRSCSIRHRMASVIFSLEMSKSEIVMRLLSAEAKIKLSDMRSGRMSDDDWTRLARRMSEISEAPLFIDDSPNLTMMEIRAKARRLRQKANLKLIVVDYLQLMTSGKKYESRQVEVSEFSRHLKLLAKELEVPVVAISQLNRGPEQRTDKKPMLADLRESGCLTASTRILRADTGAEVAFGELMRSGERPMVWSLDERLRMVARPMINVFPSGRKEVFRLRLASGREVEATGSHPFMKFEGWTPLAQLKVGDRIAAPRRVPEPIDTQRMPESELISLARMIGDGSCLKNQPIRYEPVDEANLAAVTVSAAHSDGAAIRDDYLAARVPSLRPARQRLPRGRCTPIAAWLAGLGLFTKRSHEKCVPEAVFRAPNDQVALFLRHLWSAGGSVRWDPTNGQGRVYYGSTSRRLIDDVAQLLLRVGIFSWITHAPKLGGHDSWRLHIHGAKDQVRFLRHVGVHGAEAVAAQEMLRQLKGPVRNPNLDSAPKKVWAQVRNRLSAKQMMDIQLHEPTMWKHSPSRSRPHRAEARIEDRAIHELARGDAYWDTVVEITSIGDQHVFDGTVSGTHNFVANGISLHNSLEQDADVVILLHRPDAFDRDDPRGGEADFILAKHRNGPTKTVTVAHQLHLSRFANMAR
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