Gene Mb3423
in Mycobacterium bovis AF2122/97
General annotation
| Type | CDS |
| Function | Unknown |
| Product | POSSIBLE MULTI-FUNCTIONAL ENZYME WITH ACYL-CoA-REDUCTASE ACTIVITY ACRA1 |
| Comments | Mb3423, acrA1, len: 650 aa. Equivalent to Rv3391,len: 650 aa, from Mycobacterium tuberculosis strain H37Rv,(100.0% identity in 650 aa overlap). Possible acrA1, multi functional protein with fatty acyl-CoA reductase activity in C-terminal part (EC 1.2.1.-). Indeed C-terminal part highly similar to P94129|ACR1 FATTY ACYL-COA REDUCTASE from Acinetobacter calcoaceticus (295 aa), FASTA scores: opt: 767, E(): 1.4e-36, (45.4% identity in 260 aa overlap); and similar to other oxidoreductases dehydrogenases/reductases e.g. Q9Y3A1 CGI-93 PROTEIN (SIMILARITY WITH SDR FAMILY) from Homo sapiens (Human) (291 aa), FASTA scores: opt: 363, E(): 1.5e-13, (38.65% identity in 194 aa overlap); Q9L146|SC6D11.09 PUTATIVE OXIDOREDUCTASE (SIMILARITY WITH SDR FAMILY) from Streptomyces coelicolor (343 aa), FASTA scores: opt: 346,E(): 1.6e-12, (30.4% identity in 283 aa overlap); Q9HSR4|YUSZ1|VNG0115G OXIDOREDUCTASE from Halobacterium sp. strain NRC-1 (260 aa), FASTA scores: opt: 338, E(): 3.7e-12, (33.85% identity in 248 aa overlap); etc. C-terminus also similar to Mycobacterium tuberculosis proteins Q10783|YF43_MYCTU|Rv1543|MTCY48.22c PUTATIVE OXIDOREDUCTASE (341 aa), FASTA scores: opt: 787, E(): 1.2e-37, (39.8% identity in 319 aa overlap); O06413|Rv0547c|MTCY25D10.26c HYPOTHETICAL 31.8 KDA PROTEIN (294 aa), FASTA scores: opt: 565, E(): 4.7e-25, (36.8% identity in 242 aa overlap); O53398|Rv1050|MTV017.03 OXIDOREDUCTASE (SDR FAMILY) (301 aa), FASTA scores: opt: 436, E(): 1.1e-17, (32.2% identity in 292 aa overlap). N-terminus (aa 1-320) is similar to P37693|HETM_ANASP polyketide synthase hetM from Anabaena sp. (506 aa), FASTA scores: opt: 188, E(): 1.3e-07, (27.7% identity in 361 aa overlap); so certainly a multi-domain enzyme. SEEMS TO BELONG TO THE SHORT-CHAIN DEHYDROGENASES/REDUCTASES (SDR) FAMILY. Note that this ORF corresponds to the gene ORF2|Q11197 (in citation mentioned below), but longer 266 aa, due to use of a more upstream start site. |
| Functional category | |
| Mutant | Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3757958 | 3759910 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium bovis AF2122-97|Mb3423|acrA1
MRYVVTGGTGFIGRHVVSRLLDGRPEARLWALVRRQSLSRFERLAGQWGDRVRPLVGDLTELELSERTIAELGDIDHVLHCAAVHDTTWADATRAVIELAARLDATFHHVSSIAVAGDFAGHYTEADFDVGQRLPTPYHRMTFEAERLVRSTPGLRYRIYRPAVVVGDSRTGEMDTIDGPYYLFGVLAKLAVLPSFTPMLLPDIGRTNIVPVDYVADALVALMHADGRDGQTFHLTAPTAIGLRGIYRGIAGAAGLPPLLGTLPGFVAAPVLNARGRAKVLRNMAATQLGIPAEIFDVVGCAPTFTSDTTREALRGTGIHVPEFATYAPGLWRYWAEHLDPDRARRNDPLLGRHVIITGASSGIGRASAIAVAKRGATVFALARNGNALDELVTEIRAHGGQAHAFTCDVTDSASVEHTVKDILGRFDHVDYLVNNAGRSIRRSVVNSTDRLHDYERVMAVNYFGAVRMVLALLPHWRERRFGHVVNVSSAGVQARNPKYSSYLPTKAALDAFADVVASETLSDHITFTNIHMPLVATPMIVPSRRLNPVRAISAERAAAMVIRGLVEKPARIDTPLGTLAEAGNYVAPRLSRRILHQLYLGYPDSAAAQGISRPDADRPPAPRRPRRSARAGVPRPLRRLGRLVPGVHW
Bibliography
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