Gene Mb3479
in Mycobacterium bovis AF2122/97
General annotation
| Type | CDS |
| Function | Unknown |
| Product | probable membrane-anchored mycosin mycp4 (serine protease) (subtilisin-like protease) (subtilase-like) (mycosin-4) |
| Comments | Mb3479, -, len: 455 aa. Equivalent to Rv3449, len: 455 aa, from Mycobacterium tuberculosis strain H37Rv,(100.0% identity in 455 aa overlap). Probable secreted serine protease (EC 3.4.21.-). Similar to hypothetical unknowns or proteases from Mycobacterium tuberculosis strains H37Rv and CDC1551 e.g. AAK48366|MT3998 SUBTILASE FAMILY PROTEIN from Mycobacterium tuberculosis strain CDC1551 (411 aa), FASTA scores: opt: 747, E(): 3.5e-33,(45.65% identity in 416 aa overlap); O05461|Rv3883c|MTCY15F10.29 HYPOTHETICAL PROTEIN (446 aa),FASTA scores: opt: 747, E(): 3.8e-33, (45.45% identity in 451 aa overlap); O53695|Rv0291|MTV035.19 HYPOTHETICAL PROTEIN (461 aa), FASTA scores: opt: 660, E(): 1.9e-28,(44.0% identity in 457 aa overlap); etc. And similar to hypothetical proteases from Mycobacterium leprae e.g. O33076|MLCB628.04|ML0041 HYPOTHETICAL 45.7 KDA PROTEIN (PROBABLE SECRETED PROTEASE) (446 aa), FASTA scores: opt: 683, E(): 1.1e-29, (43.8% identity in 450 aa overlap); Q9CD36|ML2528 PUTATIVE PROTEASE (475 aa), FASTA scores: opt: 608, E(): 1.3e-25, (43.0% identity in 451 aa overlap); Q9CBV3|ML1538 POSSIBLE PROTEASE (567 aa), FASTA scores: opt: 389, E(): 9.7e-14, (33.8% identity in 562 aa overlap); etc. Also some similarity to other proteases from several organisms e.g. O31788|APRX ALKALINE SERINE PROTEASE from Bacillus subtilis (442 aa), FASTA scores: opt: 296, E(): 8.3e-09, (29.4% identity in 313 aa overlap); O86650|SC3C3.17c PUTATIVE SECRETED SERINE PROTEASE from Streptomyces coelicolor (450 aa), FASTA scores: opt: 279, E(): 7e-08, (33.55% identity in 343 aa overlap); Q9KBJ7|APRX|BH193 INTRACELLULAR ALKALINE SERINE PROTEASE from Bacillus halodurans (444 aa), FASTA scores: opt: 257, E(): 1.1e-06, (28.65% identity in 335 aa overlap); O86642|SC3C3.08 SERINE PROTEASE from Streptomyces coelicolor (413 aa), FASTA scores: opt: 243,E(): 5.7e-06, (38.25% identity in 387 aa overlap); etc. Has putative signal peptide at N-terminus and hydrophobic stretch at C-terminus. Contains three signatures typical of subtilase family: aspartic acid active site (PS00136),histidine active site (PS00137), serine active site (PS00138). |
| Functional category | Intermediary metabolism and respiration |
| Mutant | Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3817453 | 3818820 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium bovis AF2122-97|Mb3479|mycp4
MTTSRTLRLLVVSALATLSGLGTPVAHAVSPPPIDERWLPESALPAPPRPTVQREVCTEVTAESGRAFGRAERSAQLADLDQVWRLTRGAGQRVAVIDTGVARHRRLPKVVAGGDYVFTGDGTADCDAHGTLVAGIIAAAPDAQSDNFSGVAPDVTLISIRQSSSKFAPVGDPSSTGVGDVDTMAKAVRTAADLGASVINISSIACVPAAAAPDDRALGAALAYAVDVKNAVIVAAAGNTGGAAQCPPQAPGVTRDSVTVAVSPAWYDDYVLTVGSVNAQGEPSAFTLAGPWVDVAATGEAVTSLSPFGDGTVNRLGGQHGSIPISGTSYAAPVVSGLAALIRARFPTLTARQVMQRIESTAHHPPAGWDPLVGNGTVDALAAVSSDSIPQAGTATSDPAPVAVPVPRRSTPGPSDRRALHTAFAGAAICLLALMATLATASRRLRPGRNGIAGD
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