Gene Rv0165c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Involved in transcriptional mechanism |
| Product | Probable transcriptional regulatory protein Mce1R (probably GntR-family) |
| Comments | Rv0165c, (MTCI28.05c), len: 223 aa. Probable mce1R, transcriptional regulator, GntR family (See Casali et al., 2006), showing some similarity to several e.g. NTRA_CHELE|P54988 nta operon transcriptional regulator (231 aa), FASTA scores: opt: 154, E(): 0.00058, (32.0% identity in 125 aa overlap); P46833|GNTR_BACLI gluconate operon transcriptional repressor from Bacillus licheniformis (243 aa); GNTR_BACSU gluconate operon repressor from Bacillus subtilis (243 aa). Also similar to Rv0043c from Mycobacterium tuberculosis. Seems to belong to the GntR family of transcriptional regulators. Start changed since first submission (-41 aa). |
| Functional category | Regulatory proteins |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). RT-qPCR shows higher expression of fadD5|Rv0166, mce1A|Rv0169 and mce1F|Rv0174 in M. tuberculosis H37Rv mce1R|Rv0165 mutant than in H37Rv, after infection of murine macrophages (See Casali et al., 2006). M. tuberculosis H37Rv mce1R|Rv0165 mutant has increased virulence in mice (See Uchida et al., 2007). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 194144 | 194815 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv0165c|mce1R
VNAPLSAKPRSQLPLRRAQLSDEVAGHLRAAIMSGALRSGTFIRLDETAAELGVSVTPVREALLKLRGEGMVGLEPHRGHVVLPLTRQDIDDIFWLQATIAQELATSATAHITDVEIDELDRINNALAGAIGSGDAKTIASIEFAFHRVFNKASRRIKLAWFLLNAARYMGAGVRGRPAMGRGRGEQSSAADRRAAPPRHSRRNRAHRLAVHRWGTQADGGPG
Bibliography
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Casali N, White AM and Riley LW [2006]. Regulation of the Mycobacterium tuberculosis mce1 operon. Mutant Product
- Uchida Y, Casali N, White A, Morici L, Kendall LV and Riley LW [2007]. Accelerated immunopathological response of mice infected with Mycobacterium tuberculosis disrupted in the mce1 operon negative transcriptional regulator. Mutant
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
