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virulence, detoxification, adaptation
information pathways
cell wall and cell processes
stable RNAs
insertion seqs and phages
PE/PPE
intermediary metabolism and respiration
unknown
regulatory proteins
conserved hypotheticals
lipid metabolism
pseudogenes
General annotation
TypeCDS
FunctionInvolved in thiamine biosynthesis. Required for the synthesis of the thiazole moiety of thiamine.
ProductProbable thiamin biosynthesis protein ThiG (thiazole biosynthesis protein)
CommentsRv0417, (MTCY22G10.14), len: 252 aa. Probable thiG, thiamin biosynthesis protein, equivalent to AL035159|MLCB1450_20|T44741|THIG probable thiamin biosynthesis protein from Mycobacterium leprae (261 aa), FASTA scores: opt: 1380, E(): 0, (86.8% identity in 250 aa overlap). Also highly similar to others e.g. SCOEDB|SC6E10.03|T35490|THIG probable thiazole biosynthesis protein from Streptomyces coelicolor (264 aa); F82761|9105679|AAF83593.1|AE003919_4|XF0783|THIG thiamin biosynthesis protein thiG from Xylella fastidiosa (275 aa); P30139|THIG_ECOLI|7448315|B65206|409790|AAC43089.1|U00006 THIG protein thiamin biosynthesis protein from Escherichia coli strain K-12 (281 aa); etc. Belongs to the THIG family.
Functional categoryIntermediary metabolism and respiration
ProteomicsIdentified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in the membrane protein fraction and whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate (See de Souza et al., 2011).
MutantEssential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011).
Check for mutants available at TARGET website
Coordinates
TypeStartEndOrientation
CDS502366503124+
Genomic sequence
Feature type Upstream flanking region (bp) Downstream flanking region (bp) Update
       
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv0417|thiG
VAESKLVIGDRSFASRLIMGTGGATNLAVLEQALIASGTELTTVAIRRVDADGGTGLLDLLNRLGITPLPNTAGSRSAAEAVLTAQLAREALNTNWVKLEVIADERTLWPDAVELVRAAEQLVDDGFVVLPYTTDDPVLARRLEDTGCAAVMPLGSPIGTGLGIANPHNIEMIVAGARVPVVLDAGIGTASDAALAMELGCDAVLLASAVTRAADPPAMAAAMAAAVTAGYLARCAGRIPKRFWAQASSPAR