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virulence, detoxification, adaptation
information pathways
cell wall and cell processes
stable RNAs
insertion seqs and phages
PE/PPE
intermediary metabolism and respiration
unknown
regulatory proteins
conserved hypotheticals
lipid metabolism
pseudogenes
General annotation
TypeCDS
FunctionThought to be involved in glyoxal pathway. Thiolesterase that catalyses the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid [catalytic activity: (S)-(2-hydroxyacyl)glutathione + H2O = glutathione + a 2-hydroxy acid anion].
ProductPossible glyoxalase II (hydroxyacylglutathione hydrolase) (GLX II)
CommentsRv0634c, (MTCY20H10.15c), len: 237 aa. Possible glyoxalase II, equivalent to NP_302290.1|NC_002677 putative glyoxylase II from Mycobacterium leprae (238 aa); and similar to U00011_3|Y0BK_MYCLE|Q49649 hypothetical 23.9 kDa protein from Mycobacterium leprae (218 aa), FASTA scores: opt: 281, E(): 3.9e-12, (31.8% identity in 201 aa overlap). Also similar to other glyoxalases and metallo-beta-lactamase family proteins e.g. NP_386770.1|NC_003047 putative hydroxyacylglutathione hydrolase from Sinorhizobium meliloti (256 aa); etc. Also similar to other putative glyoxylases from Mycobacterium tuberculosis e.g. Rv1637c. Belongs to the glyoxalase II family. Cofactor: binds two zinc ions.
Functional categoryVirulence, detoxification, adaptation
ProteomicsIdentified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in the membrane protein fraction and whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate (See de Souza et al., 2011).
MutantNon-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011).
Check for mutants available at TARGET website
Coordinates
TypeStartEndOrientation
CDS730320731033-
Genomic sequence
Feature type Upstream flanking region (bp) Downstream flanking region (bp) Update
       
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv0634c|Rv0634c
VSKDRLYFRQLLSGRDFAVGDMFATQMRNFAYLIGDRTTGDCVVVDPAYAAGDLLDALESDDMQLSGVLVTHHHPDHVGGSMMGFQLPGLAELLERASVPVHVNTHEALWVSRVTGIPVGDLITHEHGDKVSVGDIDIELLHTPGHTPGSQCFLLDGRLVAGDTLFLEGCGRTDFPGGDSDEMYRSLRQLAELPGDPTVFPGHWYSAEPSASLSEVKRSNYVYRPASLDQWRMLMGG