Gene Rv0693 (pqqIII)
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Required for coenzyme pyrrolo-quinoline-quinone (PQQ) biosynthesis. |
| Product | Probable coenzyme PQQ synthesis protein E PqqE (coenzyme PQQ synthesis protein III) |
| Comments | Rv0693, (MTCY210.10), len: 391 aa. Probable pqqE (alternate gene name: pqqIII), coenzyme PQQ synthesis protein E, similar to others AE001109_9|O30258|PQQE coenzyme PQQ synthesis protein from Archaeoglobus fulgidus (375 aa), FASTA scores: E(): 1.6e-16, (28.1% identity in 377 aa overlap); PQQE_ACICA|P07782 coenzyme pqq synthesis protein e from Acinetobacter calcoaceticus (384 aa), FASTA scores: opt: 302, E(): 1.8e-12, (23.9% identity in 377 aa overlap); etc. Also similar to C-terminus of heme biosynthesis proteins e.g. O28270|AF2009 heme biosynthesis protein (NIRJ-2) from Archaeoglobus fulgidus (468 aa). Note that also highly similar to U17129|RSU17129_4|AAC77478.1 unknown protein from Rhodococcus erythropolis (405 aa), FASTA scores: opt: 1997, E(): 0, (73.3% identity in 390 aa overlap). Could belong to the MoaA / NifB / PqqE family. |
| Functional category | Intermediary metabolism and respiration |
| Proteomics | Identified in the cell membrane fraction of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Non-essential gene for in vitro growth of H37Rv, but essential for in vitro growth on cholesterol; by sequencing of Himar1-based transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 792157 | 793332 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv0693|pqqE
MTSPVPRLIEQFERGLDAPICLTWELTYACNLACVHCLSSSGKRDPGELSTRQCKDIIDELERMQVFYVNIGGGEPTVRPDFWELVDYATAHHVGVKFSTNGVRITPEVATRLAATDYVDVQISLDGATAEVNDAIRGTGSFDMAVRALQNLAAAGFAGVKISVVITRRNVAQLDEFATLASRYGATLRITRLRPSGRGTDVWADLHPTADQQVQLYDWLVSKGERVLTGDSFFHLAPLGQSGALAGLNMCGAGRVVCLIDPVGDVYACPFAIHDHFLAGNVLSDGGFQNVWKNSSLFRELREPQSAGACGSCGHYDSCRGGCMAAKFFTGLPLDGPDPECVQGHSEPALARERHLPRPRADHSRGRRVSKPVPLTLSMRPPKRPCNESPV
Bibliography
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Mawuenyega KG et al. [2005]. Mycobacterium tuberculosis functional network analysis by global subcellular protein profiling. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
