Gene Rv0711
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Thought to play an important role in the mineralization of sulfates [catalytic activity: a phenol sulfate + H2O = a phenol + sulfate]. |
| Product | Possible arylsulfatase AtsA (aryl-sulfate sulphohydrolase) (arylsulphatase) |
| Comments | Rv0711, (MTCY210.30), len: 787 aa. Possible atsA, arylsulfatase, similar to others e.g. P51691|ARS_PSEAE arylsulfatase from Pseudomonas aeruginosa (532 aa), FASTA scores: opt: 439, E(): 2.9e-21, (30.8% identity in 552 aa overlap); etc. Also similar to other hypothetical arylsulfatases from Mycobacterium tuberculosis e.g. Rv3299c, Rv0663, etc. Contains PS00523 Sulfatases signature 1, and PS00149 Sulfatases signature 2. Belongs to the sulfatase family. |
| Functional category | Intermediary metabolism and respiration |
| Proteomics | Identified in the cell membrane fraction of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). Identified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in M. tuberculosis H37Rv-infected guinea pig lungs at 30 days but not 90 days (See Kruh et al., 2010). Identified by mass spectrometry in the culture filtrate, membrane protein fraction, and whole cell lysates of M. tuberculosis H37Rv (See de Souza et al., 2011). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv and CDC1551 strains (see Sassetti et al., 2003 and Lamichhane et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 806335 | 808698 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv0711|atsA
MAPEATEAFNGTIELDIRDSEPDWGPYAAPVAPEHSPNILYLVWDDVGIATWDCFGGLVEMPAMTRVAERGVRLSQFHTTALCSPTRASLLTGRNATTVGMATIEEFTDGFPNCNGRIPADTALLPEVLAEHGYNTYCVGKWHLTPLEESNMASTKRHWPTSRGFERFYGFLGGETDQWYPDLVYDNHPVSPPGTPEGGYHLSKDIADKTIEFIRDAKVIAPDKPWFSYVCPGAGHAPHHVFKEWADRYAGRFDMGYERYREIVLERQKALGIVPPDTELSPINPYLDVPGPNGETWPLQDTVRPWDSLSDEEKKLFCRMAEVFAGFLSYTDAQIGRILDYLEESGQLDNTIIVVISDNGASGEGGPNGSVNEGKFFNGYIDTVAESMKLFDHLGGPQTYNHYPIGWAMAFNTPYKLFKRYASHEGGIADPAIISWPNGIAAHGEIRDNYVNVSDITPTVYDLLGMTPPGTVKGIPQKPMDGVSFIAALADPAADTGKTTQFYTMLGTRGIWHEGWFANTIHAATPAGWSNFNADRWELFHIAADRSQCHDLAAEHPDKLEELKALWFSEAAKYNGLPLADLNLLETMTRSRPYLVSERASYVYYPDCADVGIGAAVEIRGRSFAVLADVTIDTTGAEGVLFKHGGAHGGHVLFVRDGRLHYVYNFLGERQQLVSSSGPVPSGRHLLGVRYLRTGTVPNSHTPVGDLELFFDENLVGALTNVLTHPGTFGLAGAAISVGRNGGSAVSSHYEAPFAFTGGTITQVTVDVSGRPFEDVESDLALAFSRD
Bibliography
- Lamichhane G et al. [2003]. A postgenomic method for predicting essential genes at subsaturation levels of mutagenesis: application to Mycobacterium tuberculosis. Mutant
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Mawuenyega KG et al. [2005]. Mycobacterium tuberculosis functional network analysis by global subcellular protein profiling. Proteomics
- Rodrigue S et al. [2007]. Identification of mycobacterial sigma factor binding sites by chromatin immunoprecipitation assays. Regulon
- Målen H et al. [2010]. Definition of novel cell envelope associated proteins in Triton X-114 extracts of Mycobacterium tuberculosis H37Rv. Proteomics
- Kruh NA et al. [2010]. Portrait of a pathogen: the Mycobacterium tuberculosis proteome in vivo. Proteomics
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
