Gene Rv0772
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Involved in de novo purine biosynthesis (at the second step) [catalytic activity: ATP + 5-phosphoribosylamine + glycine = ADP + phosphate + 5'-phosphoribosylglycinamide]. |
| Product | Probable phosphoribosylamine--glycine ligase PurD (GARS) (glycinamide ribonucleotide synthetase) (phosphoribosylglycinamide synthetase) (5'-phosphoribosylglycinamide synthetase) |
| Comments | Rv0772, (MTCY369.17), len: 422 aa. Probable purD, phosphoribosylamine--glycine ligase, equivalent to Q50144|PURD|PUR2_MYCLE|ML2235|MLCB5.08 phosphoribosylamine--glycine ligase from Mycobacterium leprae (422 aa), FASTA scores: opt: 2272, E(): 0, (81.8% identity in 422 aa overlap). Also highly similar to others e.g. CAB56348.1|AL118514 phosphoribosylamine-glycine ligase from Streptomyces coelicolor (416 aa); P1564|PUR2_ECOLI phosphoribosylamine--glycine ligase from Escherichia coli (429 aa), FASTA scores: opt: 1039, E(): 0, (42.7% identity in 431 aa overlap); etc. Belongs to the GarS family. |
| Functional category | Intermediary metabolism and respiration |
| Proteomics | Identified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in the culture filtrate, membrane protein fraction, and whole cell lysates of M. tuberculosis H37Rv (See de Souza et al., 2011). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 864586 | 865854 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv0772|purD
VRVLVIGSGAREHALLLALGKDPQVSGLIVAPGNAGTARIAEQHDVDITSAEAVVALAREVGADMVVIGPEVPLVLGVADAVRAAGIVCFGPGKDAARIEGSKAFAKDVMAAAGVRTANSEIVDSPAHLDAALDRFGPPAGDPAWVVKDDRLAAGKGVVVTADRDVARAHGAALLEAGHPVLLESYLDGPEVSLFCVVDRTVVVPLLPAQDFKRVGEDDTGLNTGGMGAYAPLPWLPDNIYREVVSRIVEPVAAELVRRGSSFCGLLYVGLAITARGPAVVEFNCRFGDPETQAVLALLESPLGQLLHAAATGKLADFGELRWRDGVAVTVVLAAENYPGRPRVGDVVVGSEAEGVLHAGTTRRDDGAIVSSGGRVLSVVGTGADLSAARAHAYEILSSIRLPGGHFRSDIGLRAAEGKISV
Bibliography
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Målen H et al. [2010]. Definition of novel cell envelope associated proteins in Triton X-114 extracts of Mycobacterium tuberculosis H37Rv. Proteomics
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
