Gene Rv0783c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Translocase that confers resistance to substances of high hydrophobicity. Involved in transport of multidrug across the membrane (export): multidrug resistance by an export mechanism. Responsible for the translocation of the substrate across the membrane. |
| Product | Possible multidrug resistance integral membrane efflux protein EmrB |
| Comments | Rv0783c, (MTCY369.27c), len: 540 aa. Possible emrB, integral membrane drug efflux protein, member of major facilitator superfamily (MFS), equivalent to AAL16083.1|AF421382_1|AF421382 EmrB efflux protein from Mycobacterium avium (538 aa). Also similar to other membrane proteins e.g. CAB61606.1|AL133210 putative export protein from Streptomyces coelicolor (496 aa); NP_108371.1|NC_002678 efflux pump protein FarB from Mesorhizobium loti (511 aa); P44927|EMRB_HAEINHI0897| multidrug resistance protein b homologue from Haemophilus influenzae (510 aa), FASTA scores: opt: 706, E(): 1.3e-36, (30.4% identity in 408 aa overlap); etc. Also similar to Rv2333c|MTCY3G12.01 from Mycobacterium tuberculosis (537 aa), FASTA score: (28.2% identity in 408 aa overlap); and Rv1410c|MTCY21B4.27c from Mycobacterium tuberculosis (518 aa), FASTA score: (26.8% identity in 496 aa overlap). Belongs to the major facilitator family; also known as the drug resistance translocase family. |
| Functional category | Cell wall and cell processes |
| Proteomics | Identified by mass spectrometry in M. tuberculosis H37Rv-infected guinea pig lungs at 90 days but not 30 days (See Kruh et al., 2010). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 876818 | 878440 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv0783c|emrB
MLGNAMVEACPAEGDAPVPITPAGRPRSGQRSYPDRLDVGLLRTAGVCVLASVMAHVDVTVVSVAQRTFVADFGSTQAVVAWTMTGYMLALATVIPTAGWAADRFGTRRLFMGSVLAFTLGSLLCAVAPNILLLIIFRVVQGFGGGMLTPVSFAILAREAGPKRLGRVMAVVGIPMLLGPVGGPILGGWLIGAYGWRWIFLVNLPVGLSALVLAAIVFPRDRPAASENFDYMGLLLLSPGLATFLFGVSSSPARGTMADRHVLIPAITGLALIAAFVAHSWYRTEHPLIDMRLFQNRAVAQANMTMTVLSLGLFGSFLLLPSYLQQVLHQSPMQSGVHIIPQGLGAMLAMPIAGAMMDRRGPAKIVLVGIMLIAAGLGTFAFGVARQADYLPILPTGLAIMGMGMGCSMMPLSGAAVQTLAPHQIARGSTLISVNQQVGGSIGTALMSVLLTYQFNHSEIIATAKKVALTPESGAGRGAAVDPSSLPRQTNFAAQLLHDLSHAYAVVFVIATALVVSTLIPAAFLPKQQASHRRAPLLSA
Bibliography
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Kruh NA et al. [2010]. Portrait of a pathogen: the Mycobacterium tuberculosis proteome in vivo. Proteomics
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
