Gene Rv0823c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Thought to be involved in transcriptional mechanism. |
| Product | Possible transcriptional regulatory protein |
| Comments | Rv0823c, (MTV043.15c), len: 389 aa. Possible transcriptional regulator (resembles nitrogen regulation protein), equivalent (but longer 24 aa in N-terminus) to MLU15182_31|U15182|NtrB NtrB protein from Mycobacterium leprae (384 aa), FASTA scores: opt: 2070, E(): 0, (82.3% identity in 384 aa overlap) (see citation below). Also highly similar to CAB63312.1|AL133471|SCC82.03c hypothetical protein from Streptomyces coelicolor (406 aa); and to many transcriptional regulators members of UPF0034 family (NIFR3/SMM1) e.g. D26185|BAC180K_143 protein similar to transcriptional regulator (nitrogen regulation protein) from Bacillus subtilis (333 aa), FASTA scores: opt: 609, E(): 1.4e-32, (38.3% identity in 326 aa overlap); NP_349795.1|NC_003030 NifR3 family enzyme from Clostridium acetobutylicum (321 aa); etc. Contains PS01136 Uncharacterized protein family UPF0034 signature. |
| Functional category | Regulatory proteins |
| Proteomics | Identified by mass spectrometry in M. tuberculosis H37Rv-infected guinea pig lungs at 30 days but not 90 days (See Kruh et al., 2010). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Disruption of this gene provides a growth advantage for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv and CDC1551 strains (see Sassetti et al., 2003 and Lamichhane et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 916477 | 917646 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv0823c|Rv0823c
MSRRRAIQPSPALRIGPIELASPVVLAPMAGVTNVAFRALCRQLEQSKVGTVSGLYVCEMVTARALIERHPVTMHMTTFSADESPRSLQLYTVDPDTTYAAARMIAGEGLADHIDMNFGCPVPKVTKRGGGAALPFKRRLFGQIVAAAVRATEGTDIPVTVKFRIGIDDAHHTHLDAGRIAEAEGAAAVALHARTAAQRYSGTADWEQIARLKQHVRTIPVLGNGDIYDAGDALAMMSTTGCDGVVIGRGCLGRPWLFAELSAAFTGSPAPTPPTLGEVADIIRRHGTLLAAHFGEDKGMRDIRKHIAWYLHGFPAGSALRRALAMVKTFDELDCLLDRLDGTVPFPDSATGARGRQGSPARVALPDGWLTDPDDCRVPEGADAMGSGG
Bibliography
- Jackson M et al. [1997]. Mycobacterium tuberculosis Des protein: an immunodominant target for the humoral response of tuberculous patients. Secondary
- Lamichhane G et al. [2003]. A postgenomic method for predicting essential genes at subsaturation levels of mutagenesis: application to Mycobacterium tuberculosis. Mutant
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Arnvig KB et al. [2009]. Identification of small RNAs in Mycobacterium tuberculosis. Operon
- Kruh NA et al. [2010]. Portrait of a pathogen: the Mycobacterium tuberculosis proteome in vivo. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
