Gene Rv0899 (ompATb)
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | The protein behaved as a porin of low specific activity. Structural protein that may protect the integrity of the bacterium. |
| Product | Outer membrane protein A OmpA |
| Comments | Rv0899, (MTCY31.27), len: 326 aa. OmpA, outer membrane protein A (See Senaratne et al., 1998). C-terminal region similar to C-terminus of many members of the OmpA family of outer membrane proteins, e.g. NP_458280.1|NC_003198 putative outer membrane protein from Salmonella enterica subsp. enterica serovar Typhi (220); NP_418008.1|NC_000913 putative outer membrane protein from Escherichia coli strain K12 (219 aa), FASTA scores: opt: 296, E(): 2.2e-11, (45.3% identity in 117 aa overlap); NP_231844.1|NC_002505 outer membrane protein OmpA from Vibrio cholerae (321 aa); Q05146|OMPA_BORAV outer membrane protein A precursor from Bordetella avium (194 aa); etc. A signal peptide sequence probably exists at the N-terminus. N-terminal domain is necessary and sufficient for membrane translocation (See Alahari et al., 2007). Contains PS00044 Bacterial regulatory proteins, lysR family signature. Belongs to the OmpA family. Pore-forming activity is pH-dependent. |
| Functional category | Cell wall and cell processes |
| Proteomics | Identified in the membrane fraction of M. tuberculosis H37Rv using nanoLC-MS/MS; predicted integral membrane protein (See Xiong et al., 2005). Putative glycoprotein identified by LC/ESI-MS/MS in the culture filtrate of M. tuberculosis H37Rv (See Gonzalez-Zamorano et al., 2009). Identified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in the culture filtrate, membrane protein fraction, and whole cell lysates of M. tuberculosis H37Rv (See de Souza et al., 2011). |
| Transcriptomics | mRNA identified by RT-PCR (see citation below). Real-time PCR shows increased transcription during growth of M. tuberculosis H37Rv (streptomycin-resistant strain 1424) in low pH media, THP1 human monocytes, and murine bone marrow macrophages (See Raynaud et al., 2002). |
| Mutant | Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). M. tuberculosis H37Rv (streptomycin-resistant strain 1424) ompA mutant shows impaired growth in low pH media, THP1 human monocytes, and murine bone marrow macrophages; mutant has reduced virulence in Balb/c mice (See Raynaud et al., 2002). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 1002812 | 1003792 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv0899|ompA
VASKAGLGQTPATTDARRTQKFYRGSPGRPWLIGAVVIPLLIAAIGYGAFERPQSVTGPTGVLPTLTPTSTRGASALSLSLLSISRSGNTVTLIGDFPDEAAKAALMTALNGLLAPGVNVIDQIHVDPVVRSLDFSSAEPVFTASVPIPDFGLKVERDTVTLTGTAPSSEHKDAVKRAATSTWPDMKIVNNIEVTGQAPPGPPASGPCADLQSAINAVTGGPIAFGNDGASLIPADYEILNRVADKLKACPDARVTINGYTDNTGSEGINIPLSAQRAKIVADYLVARGVAGDHIATVGLGSVNPIASNATPEGRAKNRRVEIVVN
Bibliography
- Senaratne RH et al. [1998]. Expression of a gene for a porin-like protein of the OmpA family from Mycobacterium tuberculosis H37Rv. Product Biochemistry
- Raynaud C et al. [2002]. The functions of OmpATb, a pore-forming protein of Mycobacterium tuberculosis. Function Mutant Transcriptome
- Xiong Y, Chalmers MJ, Gao FP, Cross TA and Marshall AG [2005]. Identification of Mycobacterium tuberculosis H37Rv integral membrane proteins by one-dimensional gel electrophoresis and liquid chromatography electrospray ionization tandem mass spectrometry. Proteomics
- Molle V et al. [2006]. pH-dependent pore-forming activity of OmpATb from Mycobacterium tuberculosis and characterization of the channel by peptidic dissection. Function
- Alahari A et al. [2007]. The N-terminal domain of OmpATb is required for membrane translocation and pore-forming activity in mycobacteria. Function
- González-Zamorano M et al. [2009]. Mycobacterium tuberculosis glycoproteomics based on ConA-lectin affinity capture of mannosylated proteins. Proteomics
- Målen H et al. [2010]. Definition of novel cell envelope associated proteins in Triton X-114 extracts of Mycobacterium tuberculosis H37Rv. Proteomics
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
